Isolation and characterization of eight microsatellite loci from Galeocerdo cuvier (tiger shark) and cross-amplification in Carcharhinus leucas, Carcharhinus brevipinna, Carcharhinus plumbeus and Sphyrna lewini

Type Article
Date 2016-05
Language English
Author(s) Pirog Agathe1, Jaquemet Sebastien1, Blaison Antonin1, 2, Soria Marc2, Magalon Helene1
Affiliation(s) 1 : Univ Reunion, UMR ENTROPIE, St Denis, Reunion, France.
2 : IRD Reunion, UMR MARBEC, St Denis, Reunion, France.
Source Peerj (2167-8359) (Peerj Inc), 2016-05 , Vol. 4 , P. e2041 (9p.)
DOI 10.7717/peerj.2041
WOS© Times Cited 10
Keyword(s) Carcharhiniform, Microsatellites, Control region, Population Genetics
Abstract

The tiger shark Galeocerdo cuvier (Carcharhinidae) is a large elasmobranch suspected to have, as other apex predators, a keystone function in marine ecosystems and is currently considered Near Threatened (Red list IUCN). Knowledge on its ecology, which is crucial to design proper conservation and management plans, is very scarce. Here we describe the isolation of eight polymorphic microsatellite loci using 454 GS-FLX Titanium pyrosequencing of enriched DNA libraries. Their characteristics were tested on a population of tiger shark (n = 101) from Reunion Island (South-Western Indian Ocean). All loci were polymorphic with a number of alleles ranging from two to eight. No null alleles were detected and no linkage disequilibrium was detected after Bonferroni correction. Observed and expected heterozygosities ranged from 0.03 to 0.76 and from 0.03 to 0.77, respectively. No locus deviated from Hardy-Weinberg equilibrium and the global F-IS of the population was of 0.04(NS). Some of the eight loci developed here successfully cross-amplified in the bull shark Carcharhinus leucas (one locus), the spinner shark Carcharhinus brevi pi n n a (four loci), the sandbar shark Carcharhinus plumbeus (five loci) and the scalloped hammerhead shark Sphyrna lewini (two loci). We also designed primers to amplify and sequence a mitochondrial marker, the control region. We sequenced 862 bp and found a low genetic diversity, with four polymorphic sites, a haplotype diversity of 0.15 and a nucleotide diversity of 2 x 10(-4).

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Dataset Microsatellite data and sequences of the control region for individuals studied. (−) indicates no data 23 KB Open access
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Pirog Agathe, Jaquemet Sebastien, Blaison Antonin, Soria Marc, Magalon Helene (2016). Isolation and characterization of eight microsatellite loci from Galeocerdo cuvier (tiger shark) and cross-amplification in Carcharhinus leucas, Carcharhinus brevipinna, Carcharhinus plumbeus and Sphyrna lewini. Peerj, 4, e2041 (9p.). Publisher's official version : https://doi.org/10.7717/peerj.2041 , Open Access version : https://archimer.ifremer.fr/doc/00626/73769/