Cultures of Dinophysis sacculus, D. acuminata and pectenotoxin 2 affect gametes and fertilization success of the Pacific oyster, Crassostrea gigas

Type Article
Date 2020-10
Language English
Author(s) Gaillard SylvainORCID1, Le Goïc Nelly4, Malo Florent1, 2, Boulais Myrina, Fabioux Caroline3, Zaccagnini Lucas4, Carpentier Liliane1, Sibat ManoellaORCID1, Réveillon DamienORCID1, Séchet VeroniqueORCID1, Hess PhilippORCID1, Hégaret HeleneORCID4
Affiliation(s) 1 : IFREMER, DYNECO, Laboratoire Phycotoxines, F-44000, Nantes, France
2 : Univ Brest, CNRS, IRD, Ifremer, LEMAR, F-29280, Plouzané, France
3 : Univ Brest, CNRS, IRD, Ifremer, LEMAR, F-29280, Plouzané, France
4 : Univ Brest, CNRS, IRD, Ifremer, LEMAR, F-29280, Plouzané, France
Source Environmental Pollution (0269-7491) (Elsevier BV), 2020-10 , Vol. 265 , N. Part B , P. 114840 (10p.)
DOI 10.1016/j.envpol.2020.114840
WOS© Times Cited 4
Keyword(s) Dinophysis spp., Okadaic acid, Pectenotoxins, Oyster gametes, Fertilization success
Abstract

Harmful algal blooms (HABs) of toxic species of the dinoflagellate genus Dinophysis are a threat to human health as they are mainly responsible for diarrheic shellfish poisoning (DSP) in the consumers of contaminated shellfish. Such contamination leads to shellfish farm closures causing major economic and social issues. The direct effects of numerous HAB species have been demonstrated on adult bivalves, whereas the effects on critical early life stages remain relatively unexplored. The present study aimed to determine the in vitro effects of either cultivated strains of D. sacculus and D. acuminata isolated from France or their associated toxins (i.e. okadaic acid (OA) and pectenotoxin 2 (PTX2)) on the quality of the gametes of the Pacific oyster Crassostrea gigas. This was performed by assessing the ROS production and viability of the gametes using flow cytometry, and fertilization success using microscopic counts. Oocytes were more affected than spermatozoa and their mortality and ROS production increased in the presence of D. sacculus and PTX2, respectively. A decrease in fertilization success was observed at concentrations as low as 0.5 cell mL−1 of Dinophysis spp. and 5 nM of PTX2, whereas no effect of OA could be observed. The effect on fertilization success was higher when both gamete types were concomitantly exposed compared to separate exposures, suggesting a synergistic effect. Our results also suggest that the effects could be due to cell-to-cell contact. These results highlight a potential effect of Dinophysis spp. and PTX2 on reproduction and recruitment of the Pacific oyster.

Full Text
File Pages Size Access
Author's final draft 50 599 KB Open access
7 581 KB Access on demand
10 848 KB Access on demand
Top of the page

How to cite 

Gaillard Sylvain, Le Goïc Nelly, Malo Florent, Boulais Myrina, Fabioux Caroline, Zaccagnini Lucas, Carpentier Liliane, Sibat Manoella, Réveillon Damien, Séchet Veronique, Hess Philipp, Hégaret Helene (2020). Cultures of Dinophysis sacculus, D. acuminata and pectenotoxin 2 affect gametes and fertilization success of the Pacific oyster, Crassostrea gigas. Environmental Pollution, 265(Part B), 114840 (10p.). Publisher's official version : https://doi.org/10.1016/j.envpol.2020.114840 , Open Access version : https://archimer.ifremer.fr/doc/00630/74167/