Genetic diversity and population structure of Tenacibaculum maritimum, a serious bacterial pathogen of marine fish: from genome comparisons to high throughput MALDI-TOF typing
Type | Article |
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Date | 2020-05 |
Language | English |
Author(s) | Bridel Sébastien1, 2, 3, Bourgeon Frédéric4, Marie Arnaud2, Saulnier Denis5, Pasek Sophie6, Nicolas Pierre7, Bernardet Jean-François1, Duchaud Eric1 |
Affiliation(s) | 1 : Université Paris-Saclay, INRAE, UVSQ, VIM, 78350, Jouy-En-Josas, France 2 : Labofarm, Finalab, 22603, Loudéac, France 3 : Université de Versailles Saint-Quentin-En-Yvelines, 78180, Montigny-Le-Bretonneux, France 4 : Bio Chêne Vert, Finalab, Rue Blaise Pascal, 35220, Châteaubourg, France 5 : Ifremer, UMR EIO 241, Labex Corail, Centre du Pacifique, BP 49, Taravao, 98719, Tahiti, French Polynesia 6 : Institut de Systématique Evolution, Biodiversité, UMR 7205 Sorbonne Université MNHN CNRS EPHE, Paris, France 7 : Université Paris-Saclay, INRAE, MaIAGE, 78350, Jouy-en-Josas, France |
Source | Veterinary Research (0928-4249) (Springer Science and Business Media LLC), 2020-05 , Vol. 51 , N. 1 , P. 60 (17p.) |
DOI | 10.1186/s13567-020-00782-0 |
WOS© Times Cited | 19 |
Abstract | Tenacibaculum maritimum is responsible for tenacibaculosis, a devastating marine fish disease. This filamentous bacterium displays a very broad host range and a worldwide geographical distribution. We analyzed and compared the genomes of 25 T. maritimum strains, including 22 newly draft-sequenced genomes from isolates selected based on available MLST data, geographical origin and host fish. The genome size (~3.356 Mb in average) of all strains is very similar. The core genome is composed of 2116 protein-coding genes accounting for ~75% of the genes in each genome. These conserved regions harbor a moderate level of nucleotide diversity (~0.0071 bp−1) whose analysis reveals an important contribution of recombination (r/m ≥ 7) in the evolutionary process of this cohesive species that appears subdivided into several subgroups. Association trends between these subgroups and specific geographical origin or ecological niche remains to be clarified. We also evaluated the potential of MALDI-TOF-MS to assess the variability between T. maritimum isolates. Using genome sequence data, several detected mass peaks were assigned to ribosomal proteins. Additionally, variations corresponding to single or multiple amino acid changes in several ribosomal proteins explaining the detected mass shifts were identified. By combining nine polymorphic biomarker ions, we identified combinations referred to as MALDI-Types (MTs). By investigating 131 bacterial isolates retrieved from a variety of isolation sources, we identified twenty MALDI-Types as well as four MALDI-Groups (MGs). We propose this MALDI-TOF-MS Multi Peak Shift Typing scheme as a cheap, fast and an accurate method for screening T. maritimum isolates for large-scale epidemiological surveys. |
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