Removal of pathogens by ultrafiltration from sea water

Type Article
Date 2020-09
Language English
Author(s) Cordier Clémence1, Stavrakakis ChristopheORCID2, Morga BenjaminORCID3, Degrémont LionelORCID3, Voulgaris Alexandra2, Bacchi Alessia2, Sauvade Patrick4, Coelho Franz4, Moulin Philippe1
Affiliation(s) 1 : Aix Marseille Univ., Laboratoire de Mécanique, Modélisation et Procédés Propres (M2P2-CNRS-UMR 7340, EPM), Equipe Procédés Membranaires, Europôle de l’Arbois, BP 80, Bat. Laennec, Hall C, 13545 Aix-en-Provence cedex 04, Laboratoire de Mécanique, Modélisation et Procédés Propres (M2P2-CNRS-UMR 7340), Aix-Marseille Université, Europôle de l’Arbois, BP 80, Bat. Laennec, Hall C, 13545 Aix-en-Provence cedex 04, France
2 : Plateforme expérimentale Mollusques Marins, Station Ifremer de Bouin, Polder des Champs, 85230 Bouin, France
3 : Laboratoire de Génétique et de Pathologie des Mollusques Marins, Station Ifremer de La Tremblade, Avenue du Mus du Loup, 17 390 La Tremblade, France
4 : Suez – Aquasource, 20, Avenue Didier Daurat, 31029 Toulouse cedex 04, France
Source Environment International (0160-4120) (Elsevier BV), 2020-09 , Vol. 142 , P. 105809 (12p.)
DOI 10.1016/j.envint.2020.105809
WOS© Times Cited 16
Keyword(s) Ultrafiltration, Biosecurisation, Pathogen retention, Sea water, OsHV-1, Shellfish production

Among water treatment processes, ultrafiltration is known to be efficient for the elimination of micro-organisms (bacteria and viruses). In this study, two pathogens were targeted, a bacterium, Vibrio aestuarianus and a virus, OsHV-1, with the objective to produce high quality water from seawater, in the case of shellfish productions. The retention of those microorganisms by ultrafiltration was evaluated at labscale. In the case of OsHV-1, the protection of oysters was validated by in vivo experiments using oysters spat and larvae, both stages being highly susceptible to the virus. The oysters raised using contaminated seawater which was then subsequently treated by ultrafiltration, had similar mortality to the negative controls. In the case of V. aestuarianus, ultrafiltration allowed a high retention of the bacteria in seawater with concentrations below the detection limits of the 3 analytical methods (flow cytometry, direct seeding and seeding after filtration to 0.22 µm). Thus, the quantity of V. aestuarianus was at least, 400 times inferior to the threshold known to induce mortalities in oysters. Industrial scale experiment on a several months period confirmed the conclusion obtained at lab scale on the Vibrio bacteria retention. Indeed, no bacteria from this genus, potentially harmful for oysters, was detected in permeate and this, whatever the quality of the seawater treated and the bacteria concentration upstream of the membrane. Moreover, the resistance of the process was confirmed with a stability of hydraulic performances over time for two water qualities and even facing an algal bloom. In terms of retention and resistance, ultrafiltration process was validated for the treatment of seawater towards the targeted pathogenic microorganisms, with the aim of biosecuring shellfish productions.

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