Methanohalophilus profundi sp. nov., a methylotrophic halophilic piezophilic methanogen isolated from a deep hypersaline anoxic basin

Type Article
Date 2020-09
Language English
Author(s) L'Haridon Stephane4, Haroun Hani4, Corre Erwan2, Roussel ErwanORCID1, Chalopin MorganeORCID5, Pignet Patricia1, Balière Charlotte1, La Cono Violetta3, Jebbar Mohamed4, Yakimov Michail3, Toffin LaurentORCID1
Affiliation(s) 1 : Univ Brest (UBO), Ifremer, CNRS, Laboratoire de Microbiologie des Environnements Extrêmes, F-29280 Plouzané, France
2 : Sorbonne Université, CNRS, Station Biologique de Roscoff, Plateforme ABiMS, F-29688 Roscoff, France
3 : Institute for Coastal Marine Environment CNR, 98122 Messina, Italy
4 : Univ Brest (UBO), Ifremer, CNRS, Laboratoire de Microbiologie des Environnements Extrêmes, F-29280 Plouzané, France
5 : Univ Brest (UBO), Ifremer, CNRS, Laboratoire de Microbiologie des Environnements Extrêmes, F-29280 Plouzané, France
Source Systematic And Applied Microbiology (0723-2020) (Elsevier BV), 2020-09 , Vol. 43 , N. 5 , P. 126107 (8p.)
DOI 10.1016/j.syapm.2020.126107
WOS© Times Cited 1
Keyword(s) Methanohalophilus, Methanosarcinaceae, Methanosarcinales, Methylotrophic, Piezophilic, Deep hypersalin anoxic basin (DHAB)
Abstract

A novel anaerobic methylotrophic halophilic methanogen strain SLHTYROT was isolated from a deep hypersaline anoxic basin called “Tyro” located in the Eastern Mediterranean Sea. Cells of SLHTYROT were motile cocci. The strain SLHTYROT grew between 12 and 37 °C (optimum 30 °C), at pH between 6.5 and 8.2 (optimum pH 7.5) and salinity from 45 to 240 g L−1 NaCl (optimum 135 g L−1). Strain SLHTYROT was methylotrophic methanogen able to use methylated compounds (trimethylamine, dimethylamine, monomethylamine and methanol). Strain SLHTYROT was able to grow at in situ hydrostatic pressure and temperature conditions (35 MPa, 14 °C). Phylogenetic analysis based on 16S rRNA gene and mcrA gene sequences indicated that strain SLHTYROT was affiliated to genus Methanohalophilus within the order Methanosarcinales. It shared >99.16% of the 16S rRNA gene sequence similarity with strains of other Methanohalophilus species. Based on ANIb, AAI and dDDH measurements, and the physiological properties of the novel isolate, we propose that strain SLHTYROT should be classified as a representative of a novel species, for which the name Methanohalophilus profundi sp. nov. is proposed; the type strain is SLHTYROT (=DSM 108854 = JCM 32768 = UBOCC-M-3308).

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L'Haridon Stephane, Haroun Hani, Corre Erwan, Roussel Erwan, Chalopin Morgane, Pignet Patricia, Balière Charlotte, La Cono Violetta, Jebbar Mohamed, Yakimov Michail, Toffin Laurent (2020). Methanohalophilus profundi sp. nov., a methylotrophic halophilic piezophilic methanogen isolated from a deep hypersaline anoxic basin. Systematic And Applied Microbiology, 43(5), 126107 (8p.). Publisher's official version : https://doi.org/10.1016/j.syapm.2020.126107 , Open Access version : https://archimer.ifremer.fr/doc/00635/74744/