Stereology reading protocol when using quantitative histology for the determination of sexual maturity in fish ovaries

Other titles Protocole de lectures stéréologiques pour l’utilisation d’histologie quantitative dans la détermination des phases de maturité sexuelle chez les ovaires de poissons
Type Normative document (norm, referencial, protocol)
Date 2023-05
Language English
Author(s) Dubroca LaurentORCID1, Elie NicolasORCID3, Heude-Berthelin ClothildeORCID2, Kellner KristellORCID2, Le Meleder Anna1, Lepoittevin Mélanie2, Martin Valentine1, Nivet Timothe1, Sauger CarineORCID1, Villain-Naud Nadège2
Affiliation(s) 1 : Institut Français de Recherche pour l’Exploitation de la Mer, IFREMER, Laboratoire Ressources Halieutiques de Port-en-Bessin, 14520 Port-en-Bessin-Huppain, France
2 : University of Caen Normandy, Biologie des Organismes et Ecosystèmes Aquatiques (BOREA) MNHN, Sorbonne Université, UCN, CNRS-7208, IRD, UA, team EMERGE, esplanade de la Paix 14032 Caen, France
3 : Normandie Univ, UNICAEN, SF 4206 ICORE, CMABIO3, 14000 Caen, France
DOI 10.13155/75173
Publisher Ifremer
Version 3.4

This protocol was established for the evaluation of sexual maturity for exploited stock species, using quantitative histology (stereology). This document describes the reading method used on histological slides under the cross-platform QuPath.

          This document follows the work of different workshops that aimed to improve the compilation of data on sexual maturity. The WKMATCH (WorKshop for MATurity staging CHairs, 2012) and the WKASMSF (Workshop for Advancing Sexual Maturity Staging in Fish, 2018) defined a universal evaluation grid for sexual maturity staging of different species, including teleosteans. During the WKMATCH workshop, two main recommendations were made, underlining the need to improve and complete knowledge on sexual maturity, as well as to harmonize the practices used to determine these sexual phases.

          Another workshop, the WKMATHIS (WorKshop on sexual MATurity staging from HIStological tools) that took place in Caen in 2017, added new objectives : review the current knowledge on gonad histology, determine a sexual maturity scale at a cellular level, and histological studies should be made in order to improve the determination of sexual maturity on a macroscopic scale. 

          This protocol, drawn from these workshops and their conclusions, offers guidelines on how to read histological slides through the use of stereology. As of June 2022, this protocol is linked to the MATO (MATurité Objectif des poissons par l’histologie quantitative) project, a study restricted to female gonad and the different cellular structures found inside the ovaries for different species (Pleuronectes platessa, Lepidorhombus whiffiagonis, Lepidorhombus boscii, Mullus surmuletus and Micromesistius poutassou).

          This protocol will present reading methods that favor an objective reading of histological slides, through stereology, and will allow less experienced readers to partake in this exercise. For a detailed description of the germinal cells, refer to the lexicon of the studied specie

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Dubroca Laurent, Elie Nicolas, Heude-Berthelin Clothilde, Kellner Kristell, Le Meleder Anna, Lepoittevin Mélanie, Martin Valentine, Nivet Timothe, Sauger Carine, Villain-Naud Nadège (2023). Stereology reading protocol when using quantitative histology for the determination of sexual maturity in fish ovaries.