Role of RadA and DNA Polymerases in Recombination-Associated DNA Synthesis in Hyperthermophilic Archaea

Type Article
Date 2020-07
Language English
Author(s) Hogrel Gaelle2, 4, Lu Yang, Alexandre Nicolas2, 4, Bossé Audrey1, 2, Dulermo Remi1, 2, Ishino Sonoko3, Ishino Yoshizumi3, Flament DidierORCID1, 2
Affiliation(s) 1 : Laboratoire de Microbiologie des Environnements Extrêmes, Ifremer, CNRS, Univ Brest, 29280 Plouzané, France
2 : LIA1211 MICROBSEA, Sino-French International Laboratory of Deep-Sea Microbiology, 29280 Xiamen-Plouzané, France
3 : Department of Bioscience and Biotechnology, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka, Fukuoka 819-0395, Japan
4 : Laboratoire de Microbiologie des Environnements Extrêmes, Ifremer, CNRS, Univ Brest, 29280 Plouzané, France
Source Biomolecules (2218-273X) (MDPI AG), 2020-07 , Vol. 10 , N. 7 , P. 1045 (17p.)
DOI 10.3390/biom10071045
Note This article belongs to the Special Issue Archaea: Diversity, Metabolism and Molecular Biology
Keyword(s) homologous recombination, Archaea, DNA polymerase, recombinase, DNA repair
Abstract

Among the three domains of life, the process of homologous recombination (HR) plays a central role in the repair of double-strand DNA breaks and the restart of stalled replication forks. Curiously, main protein actors involved in the HR process appear to be essential for hyperthermophilic Archaea raising interesting questions about the role of HR in replication and repair strategies of those Archaea living in extreme conditions. One key actor of this process is the recombinase RadA, which allows the homologous strand search and provides a DNA substrate required for following DNA synthesis and restoring genetic information. DNA polymerase operation after the strand exchange step is unclear in Archaea. Working with Pyrococcus abyssi proteins, here we show that both DNA polymerases, family-B polymerase (PolB) and family-D polymerase (PolD), can take charge of processing the RadA-mediated recombination intermediates. Our results also indicate that PolD is far less efficient, as compared with PolB, to extend the invaded DNA at the displacement-loop (D-loop) substrate. These observations coincide with previous genetic analyses obtained on Thermococcus species showing that PolB is mainly involved in DNA repair without being essential probably because PolD could take over combined with additional partners.

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How to cite 

Hogrel Gaelle, Lu Yang, Alexandre Nicolas, Bossé Audrey, Dulermo Remi, Ishino Sonoko, Ishino Yoshizumi, Flament Didier (2020). Role of RadA and DNA Polymerases in Recombination-Associated DNA Synthesis in Hyperthermophilic Archaea. Biomolecules, 10(7), 1045 (17p.). Publisher's official version : https://doi.org/10.3390/biom10071045 , Open Access version : https://archimer.ifremer.fr/doc/00641/75274/