Alterocin, an antibiofilm protein secreted by Pseudoalteromonas sp. 3J6

Type Article
Date 2020-10
Language English
Author(s) Jouault Albane1, Gobet AngeliqueORCID1, 2, 3, Simon Marjolaine1, Portier Emilie1, Perennou Morgan4, Corre Erwan4, Gaillard Fanny4, Vallenet David5, Michel Gurvan2, Fleury Yannick6, Bazire Alexis1, Dufour Alain1
Affiliation(s) 1 : Université de Bretagne-Sud, EA 3884, LBCM, IUEM, F-56100 Lorient, France
2 : Sorbonne Université, CNRS, UMR8227, Integrative Biology of Marine Models (LBI2M), Station Biologique de Roscoff (SBR), F-29680 Roscoff, France
3 : Univ Montpellier, CNRS, Ifremer, IRD, MARBEC, F-34203 Sète, France
4 : Sorbonne Université, CNRS, FR2424, Station Biologique de Roscoff (SBR), F-29680 Roscoff, France
5 : LABGeM, Génomique Métabolique, CEA, Genoscope, Institut François Jacob, CNRS, Université d'Evry, Université Paris-Saclay, F-91057 Evry, France
6 : Université de Brest, EA 3884, LBCM, IUEM, IUT Quimper, F-29000 Quimper, France
Source Applied And Environmental Microbiology (0099-2240) (American Society for Microbiology), 2020-10 , Vol. 86 , N. 20 , P. e00893-20 (19p.)
DOI 10.1128/AEM.00893-20
WOS© Times Cited 2
Keyword(s) antibiofilm protein, Pseudoalteromonas, Pseudomonas aeruginosa, biofilm
Abstract

The aim was to identify and study the antibiofilm protein secreted by the marine bacterium Pseudoalteromonas sp. 3J6. The latter is active against marine and terrestrial bacteria, including Pseudomonas aeruginosa clinical strains forming different biofilm types. Several amino acid sequences were obtained from the partially purified antibiofilm protein, named alterocin. The Pseudoalteromonas sp. 3J6 genome was sequenced and a candidate alt gene was identified by comparing the genome-encoded proteins to the sequences from purified alterocin. Expressing the alt gene in another non-active Pseudoalteromonas sp. strain, 3J3, demonstrated that it is responsible for the antibiofilm activity. Alterocin is a 139-residue protein including a predicted 20-residue signal sequence, which would be cleaved off upon export by the general secretion system. No sequence homology was found between alterocin and proteins of known functions. The alt gene is not part of an operon and adjacent genes do not seem related to alterocin production, immunity or regulation, suggesting that these functions are not fulfilled by devoted proteins. During liquid growth, the alt mRNA level peaked during the stationary phase. A single promoter was experimentally identified and several inverted repeats could be binding sites for regulators. alt genes were found in about 30% of the Pseudoalteromonas genomes, and in only few instances of other marine bacteria of the Hahella and Paraglaciecola genera. Comparative genomics yielded the hypotheses that alt gene losses occurred within the Pseudoalteromonas genus. Overall, alterocin is a novel kind of antibiofilm protein of ecological and biotechnological interest.

Importance Biofilms are microbial communities that develop on solid surfaces or interfaces, and are detrimental in a number of fields including for example food industry, aquaculture, and medicine. In the latter, antibiotics are insufficient to clear biofilm infections, leading to chronic infections such as in the case of infection by Pseudomonas aeruginosa of the lungs of cystic fibrosis patients. Antibiofilm molecules are thus urgently needed to be used in conjunction with conventional antibiotics, as well as in other fields of application, especially if they are environmentally friendly molecules. Here, we describe alterocin, a novel antibiofilm protein secreted by a marine bacterium belonging to the Pseudoalteromonas genus, and its gene. Alterocin homologs were found in about 30% of Pseudoalteromonas strains, indicating that this new family of antibiofilm proteins likely plays an important albeit non-essential function in the biology of these bacteria. This work opens up the possibility of a variety of applications.

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Jouault Albane, Gobet Angelique, Simon Marjolaine, Portier Emilie, Perennou Morgan, Corre Erwan, Gaillard Fanny, Vallenet David, Michel Gurvan, Fleury Yannick, Bazire Alexis, Dufour Alain (2020). Alterocin, an antibiofilm protein secreted by Pseudoalteromonas sp. 3J6. Applied And Environmental Microbiology, 86(20), e00893-20 (19p.). Publisher's official version : https://doi.org/10.1128/AEM.00893-20 , Open Access version : https://archimer.ifremer.fr/doc/00643/75517/