A method to assess algicidal activity of microalgal extracts coupling microalgae produced in stirred closed photobioreactor operating in continuous with pulse amplitude modulated (PAM) fluorometry
|Author(s)||Menguy Eva1, Dumontet Vincent2, Coulombier Noémie3, Meriot Vincent3, Le Dean Loic1, Barthelemy Vanille1, Jauffrais Thierry1|
|Affiliation(s)||1 : Ifremer, IRD, Univ Nouvelle‐Calédonie, Univ La Réunion, CNRS, UMR 9220 ENTROPIE, 101 Promenade Roger Laroque, 98897, Noumea, New Caledonia
2 : Université Paris Saclay, CNRS, UPR 2301, ICSN, 1 Avenue de la Terrasse, F-91198 Gif-sur-Yvette, France
3 : ADECAL Technopole, 1 bis rue Berthelot, 98846, Noumea, New Caledonia
|Source||Methodsx (22150161) (Elsevier BV), 2020 , Vol. 7 , P. 101037 (8p.)|
|WOS© Times Cited||1|
|Keyword(s)||Bioassay, Cytoxicity, Microalgae, Quantum efficiency, Fv/Fm, Non photochemical quenching, NPQ, electron transport rate, Phytochemistry, Anti algal|
We describe in the present study a quick and reliable method based on chlorophyll a fluorescence to assess putative algicidal effect of different microalgal extracts. We couple microalgal production under chemostat cultivation mode to continuously produce a given microalgae species (e.g. Dunaliella salina in this study) at a stable physiological state to ease comparison between extracts tested; with a non-destructive method based on chlorophyll a fluorescence. Pulse amplitude modulated (PAM) fluorometry was used to assess over time the effect of different microalgal crude extracts on the efficiency of the photosystem II (PSII) of a tested microalgae (Dunaliella salina).
• Microalgal production at stationary phase in stirred closed photobioreactor (PBR) operating in continuous have stable photophysiological parameters, which is a prerequisite to compare the impact of different algicidal compounds.
• The combination of both methods, allows to quickly assess the algicidal effect of diverse microalgal (crude) extracts on the PSII efficiency of a tested microalgae.
• The method may be used to identify and isolate algicidal molecules affecting algal PSII using a bio-guided isolation protocol.