A method to assess algicidal activity of microalgal extracts coupling microalgae produced in stirred closed photobioreactor operating in continuous with pulse amplitude modulated (PAM) fluorometry

Type Article
Date 2020
Language English
Author(s) Menguy Eva1, Dumontet Vincent2, Coulombier Noémie3, Meriot Vincent3, Le Dean Loic1, Barthelemy Vanille1, Jauffrais ThierryORCID1
Affiliation(s) 1 : Ifremer, IRD, Univ Nouvelle‐Calédonie, Univ La Réunion, CNRS, UMR 9220 ENTROPIE, 101 Promenade Roger Laroque, 98897, Noumea, New Caledonia
2 : Université Paris Saclay, CNRS, UPR 2301, ICSN, 1 Avenue de la Terrasse, F-91198 Gif-sur-Yvette, France
3 : ADECAL Technopole, 1 bis rue Berthelot, 98846, Noumea, New Caledonia
Source Methodsx (22150161) (Elsevier BV), 2020 , Vol. 7 , P. 101037 (8p.)
DOI 10.1016/j.mex.2020.101037
WOS© Times Cited 1
Keyword(s) Bioassay, Cytoxicity, Microalgae, Quantum efficiency, Fv/Fm, Non photochemical quenching, NPQ, electron transport rate, Phytochemistry, Anti algal
Abstract

We describe in the present study a quick and reliable method based on chlorophyll a fluorescence to assess putative algicidal effect of different microalgal extracts. We couple microalgal production under chemostat cultivation mode to continuously produce a given microalgae species (e.g. Dunaliella salina in this study) at a stable physiological state to ease comparison between extracts tested; with a non-destructive method based on chlorophyll a fluorescence. Pulse amplitude modulated (PAM) fluorometry was used to assess over time the effect of different microalgal crude extracts on the efficiency of the photosystem II (PSII) of a tested microalgae (Dunaliella salina).

• Microalgal production at stationary phase in stirred closed photobioreactor (PBR) operating in continuous have stable photophysiological parameters, which is a prerequisite to compare the impact of different algicidal compounds.

• The combination of both methods, allows to quickly assess the algicidal effect of diverse microalgal (crude) extracts on the PSII efficiency of a tested microalgae.

• The method may be used to identify and isolate algicidal molecules affecting algal PSII using a bio-guided isolation protocol.

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How to cite 

Menguy Eva, Dumontet Vincent, Coulombier Noémie, Meriot Vincent, Le Dean Loic, Barthelemy Vanille, Jauffrais Thierry (2020). A method to assess algicidal activity of microalgal extracts coupling microalgae produced in stirred closed photobioreactor operating in continuous with pulse amplitude modulated (PAM) fluorometry. Methodsx, 7, 101037 (8p.). Publisher's official version : https://doi.org/10.1016/j.mex.2020.101037 , Open Access version : https://archimer.ifremer.fr/doc/00643/75524/