Antimicrobial Peptides and Ectosymbiotic Relationships: Involvement of a Novel Type IIa Crustin in the Life Cycle of a Deep-Sea Vent Shrimp

Type Article
Date 2020-07
Language English
Author(s) Le Bloa Simon1, Boidin-Wichlacz Céline2, 3, Cueff-Gauchard ValerieORCID1, Rosa Rafael Diego4, Cuvillier-Hot Virginie3, Durand Lucile1, Methou Pierre1, 5, Pradillon FlorenceORCID5, Cambon-Bonavita Marie-AnneORCID1, Tasiemski Aurélie2, 3
Affiliation(s) 1 : Ifremer, Univ. Brest, CNRS, Laboratoire de Microbiologie des Environnements Extrêmes (LM2E), Plouzané, France
2 : Univ. Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019 – UMR 9017 - CIIL - Center for Infection and Immunity of Lille, Lille, France
3 : Univ. Lille, CNRS, UMR 8198 - Evo-Eco-Paleo, Lille, France
4 : Laboratory of Immunology Applied to Aquaculture, Department of Cell Biology, Embryology and Genetics, Federal University of Santa Catarina, Florianópolis, Brazil
5 : Ifremer, Laboratoire Environnement Profond (REM/EEP/LEP), Plouzané, France
Source Frontiers In Immunology (1664-3224) (Frontiers Media SA), 2020-07 , Vol. 11 , P. 1511 (18p.)
DOI 10.3389/fimmu.2020.01511
WOS© Times Cited 5
Keyword(s) extreme, hydrothermal, symbiosis, host-microbe interaction, invertebrate immunity, crustacean
Abstract

The symbiotic shrimp Rimicaris exoculata dominates the macrofauna inhabiting the active smokers of the deep-sea mid Atlantic ridge vent fields. We investigated the nature of the host mechanisms controlling the vital and highly specialized ectosymbiotic community confined into its cephalothoracic cavity. R. exoculata belongs to the Pleocyemata, crustacean brooding eggs, usually producing Type I crustins. Unexpectedly, a novel anti-Gram-positive type II crustin was molecularly identified in R. exoculata. Re-crustin is mainly produced by the appendages and the inner surfaces of the cephalothoracic cavity, embedding target epibionts. Symbiosis acquisition and regulating mechanisms are still poorly understood. Yet, symbiotic communities were identified at different steps of the life cycle such as brooding stage, juvenile recruitment and molt cycle, all of which may be crucial for symbiotic acquisition and control. Here, we show a spatio-temporal correlation between the production of Re-crustin and the main ectosymbiosis-related life-cycle events. Overall, our results highlight (i) a novel and unusual AMP sequence from an extremophile organism and (ii) the potential role of AMPs in the establishment of vital ectosymbiosis along the life cycle of deep-sea invertebrates.

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Le Bloa Simon, Boidin-Wichlacz Céline, Cueff-Gauchard Valerie, Rosa Rafael Diego, Cuvillier-Hot Virginie, Durand Lucile, Methou Pierre, Pradillon Florence, Cambon-Bonavita Marie-Anne, Tasiemski Aurélie (2020). Antimicrobial Peptides and Ectosymbiotic Relationships: Involvement of a Novel Type IIa Crustin in the Life Cycle of a Deep-Sea Vent Shrimp. Frontiers In Immunology, 11, 1511 (18p.). Publisher's official version : https://doi.org/10.3389/fimmu.2020.01511 , Open Access version : https://archimer.ifremer.fr/doc/00643/75534/