In the main tidal energy sites like Alderney Race, turbulence intensity is high and velocity fluctuations may have a significant impact on marine turbines. To understand such phenomena better, a three-bladed turbine model is positioned in the wake of a generic wall-mounted obstacle, representative of in situ bathymetric variation. From two-dimensional Particle Image Velocimetry planes, the time-averaged velocity in the wake of the obstacle is reconstructed in the three-dimensional space. The reconstruction method is based on Proper Orthogonal Decomposition and enables access to a representation of the mean flow field and the associated shear. Then, the effect of the velocity gradient is observed on the turbine blade root force, for four turbine locations in the wake of the obstacle. The blade root force average decreases whereas its standard deviation increases when the distance to the obstacle increases. The angular distribution of this phase-averaged force is shown to be non-homogeneous, with variation of about 20% of its time-average during a turbine rotation cycle. Such force variations due to velocity shear will have significant consequences in terms of blade fatigue.