State of art and best practices for fatty acid analysis in aquatic sciences
Type | Article | ||||||||
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Date | 2020-12 | ||||||||
Language | English | ||||||||
Author(s) | Couturier Lydie1, Michel Loic![]() ![]() ![]() |
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Affiliation(s) | 1 : Univ Brest, Ifremer, CNRS, IRD, LEMAR, Plouzané F-29280, France 2 : Ifremer, Centre de Bretagne, REM/EEP, Laboratoire Environnement Profond, Plouzané 29280, France 3 : Interdisciplinary Centre of Marine and Environmental Research (CIIMAR/CIMAR), University of Porto (U.Porto), Av. General Norton de Matos s/n, Matosinhos 4450-208, Portugal 4 : Department of Process Engineering and Applied Science, Dalhousie University, 5273 DaCosta Row, Halifax, NS B3H 4R2, Canada 5 : CESAM - Centre for Environmental and Marine Studies & LAQV-REQUIMTE, Department of Chemistry, University of Aveiro, Santiago University Campus, Aveiro 3810-193, Portugal 6 : Department of Biology, Marine Biology, Ghent University, Krijgslaan 281/S8, Ghent 9000, Belgium 7 : Marine Biotechnology Department, Stazione Zoologica Anton Dohrn Napoli, Villa Comunale, Napoli, Italy 8 : Helmholtz Centre for Environmental Research – UFZ, Department Aquatic Ecosystem Analysis and Management and Department River Ecology, Brückstrasse 3a, 30114 Magdeburg, Germany 9 : Ifremer - Centre Manche Mer du Nord, Département Ressources Biologiques et Environnement, Laboratoire des Ressources Halieutiques, 150 Quai Gambetta, BP 699, Boulogne-sur-Mer 62321, France 10 : WasserCluster Lunz – Inter-University Center for Aquatic Ecosystems Research, Lunz am See 3293, Austria 11 : CSIRO Oceans and Atmosphere, GPO Box 1538, Hobart, TAS 7000, Australia 12 : Department of Ocean Sciences, Memorial University of Newfoundland, St. John's, Newfoundland A1C 5S7, Canada |
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Source | Ices Journal Of Marine Science (1054-3139) (Oxford University Press (OUP)), 2020-12 , Vol. 77 , N. 7-8 , P. 2375-2395 | ||||||||
DOI | 10.1093/icesjms/fsaa121 | ||||||||
WOS© Times Cited | 25 | ||||||||
Keyword(s) | aquatic nutrition, biomarkers, fatty acids, marine and freshwater lipids, methods, recommended practices | ||||||||
Abstract | Determining the lipid content and fatty acid (FA) composition of aquatic organisms has been of major interest in trophic ecology, aquaculture, and nutrition for over half a century. Although protocols for lipid analysis are well-described, their application to aquatic sciences often requires modifications to adapt to field conditions and to sample type. Here, we present the current state of knowledge of methods dedicated to both marine and freshwater lipid analyses, from sampling to data treatment. We review: (i) sample preservation, storage and transport protocols, and their effects on lipids, (ii) lipid extraction, separation of polar and neutral lipids, derivatization, and detection methods, and (iii) available tools for the statistical analysis of FA data. We provide recommendations for best practices in field situations and advocate for protocol standardization and interlaboratory calibration. |
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