State of art and best practices for fatty acid analysis in aquatic sciences

Type Article
Date 2020-12
Language English
Author(s) Couturier Lydie1, Michel LoicORCID2, Amaro Teresa3, Budge Suzanne M4, Da Costa Elisabete5, de Troch Marleen6, Di Dato Valeria7, Fink Patrick8, Giraldo CarolinaORCID9, Le Grand Fabienne1, Loaiza Iván6, Mathieu-Resuge Margaux10, Nichols Peter D11, Parrish Christopher C12, Sardenne Fany1, Vagner Marie1, Pernet FabriceORCID1, Soudant Philippe1, Browman Howard
Affiliation(s) 1 : Univ Brest, Ifremer, CNRS, IRD, LEMAR, Plouzané F-29280, France
2 : Ifremer, Centre de Bretagne, REM/EEP, Laboratoire Environnement Profond, Plouzané 29280, France
3 : Interdisciplinary Centre of Marine and Environmental Research (CIIMAR/CIMAR), University of Porto (U.Porto), Av. General Norton de Matos s/n, Matosinhos 4450-208, Portugal
4 : Department of Process Engineering and Applied Science, Dalhousie University, 5273 DaCosta Row, Halifax, NS B3H 4R2, Canada
5 : CESAM - Centre for Environmental and Marine Studies & LAQV-REQUIMTE, Department of Chemistry, University of Aveiro, Santiago University Campus, Aveiro 3810-193, Portugal
6 : Department of Biology, Marine Biology, Ghent University, Krijgslaan 281/S8, Ghent 9000, Belgium
7 : Marine Biotechnology Department, Stazione Zoologica Anton Dohrn Napoli, Villa Comunale, Napoli, Italy
8 : Helmholtz Centre for Environmental Research – UFZ, Department Aquatic Ecosystem Analysis and Management and Department River Ecology, Brückstrasse 3a, 30114 Magdeburg, Germany
9 : Ifremer - Centre Manche Mer du Nord, Département Ressources Biologiques et Environnement, Laboratoire des Ressources Halieutiques, 150 Quai Gambetta, BP 699, Boulogne-sur-Mer 62321, France
10 : WasserCluster Lunz – Inter-University Center for Aquatic Ecosystems Research, Lunz am See 3293, Austria
11 : CSIRO Oceans and Atmosphere, GPO Box 1538, Hobart, TAS 7000, Australia
12 : Department of Ocean Sciences, Memorial University of Newfoundland, St. John's, Newfoundland A1C 5S7, Canada
Source Ices Journal Of Marine Science (1054-3139) (Oxford University Press (OUP)), 2020-12 , Vol. 77 , N. 7-8 , P. 2375-2395
DOI 10.1093/icesjms/fsaa121
WOS© Times Cited 5
Keyword(s) aquatic nutrition, biomarkers, fatty acids, marine and freshwater lipids, methods, recommended practices
Abstract

Determining the lipid content and fatty acid (FA) composition of aquatic organisms has been of major interest in trophic ecology, aquaculture, and nutrition for over half a century. Although protocols for lipid analysis are well-described, their application to aquatic sciences often requires modifications to adapt to field conditions and to sample type. Here, we present the current state of knowledge of methods dedicated to both marine and freshwater lipid analyses, from sampling to data treatment. We review: (i) sample preservation, storage and transport protocols, and their effects on lipids, (ii) lipid extraction, separation of polar and neutral lipids, derivatization, and detection methods, and (iii) available tools for the statistical analysis of FA data. We provide recommendations for best practices in field situations and advocate for protocol standardization and interlaboratory calibration.

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Couturier Lydie, Michel Loic, Amaro Teresa, Budge Suzanne M, Da Costa Elisabete, de Troch Marleen, Di Dato Valeria, Fink Patrick, Giraldo Carolina, Le Grand Fabienne, Loaiza Iván, Mathieu-Resuge Margaux, Nichols Peter D, Parrish Christopher C, Sardenne Fany, Vagner Marie, Pernet Fabrice, Soudant Philippe, Browman Howard (2020). State of art and best practices for fatty acid analysis in aquatic sciences. Ices Journal Of Marine Science, 77(7-8), 2375-2395. Publisher's official version : https://doi.org/10.1093/icesjms/fsaa121 , Open Access version : https://archimer.ifremer.fr/doc/00651/76279/