Defense Compounds Rather Than Nutrient Availability Shape Aggressiveness Trait Variation Along a Leaf Maturity Gradient in a Biotrophic Plant Pathogen

Type Article
Date 2018-09
Language English
Author(s) Maupetit Agathe1, Larbat Romain2, Pernaci Michaël1, Andrieux Axelle1, Guinet Cécile3, Boutigny Anne-Laure3, Fabre Bénédicte1, Frey Pascal1, Halkett Fabien1
Affiliation(s) 1 : Université de Lorraine, INRA, IAM, Nancy, France
2 : Université de Lorraine, INRA, LAE, Nancy, France
3 : ANSES, Laboratoire de la Santé des Végétaux, Unité de Mycologie, Malzéville, France
Source Frontiers in Plant Science (1664-462X) (Frontiers Media SA), 2018-09 , Vol. 9 , N. 1396 , P. 13p.
DOI 10.3389/fpls.2018.01396
WOS© Times Cited 6
Keyword(s) constitutive defense, aggressiveness traits, plasticity, nutrient availability, rust fungus
Abstract

Foliar pathogens face heterogeneous environments depending on the maturity of leaves they interact with. In particular, nutrient availability as well as defense levels may vary significantly, with opposing effects on the success of infection. The present study tested which of these factors have a dominant effect on the pathogen’s development. Poplar leaf disks of eight maturity levels were inoculated with the poplar rust fungus Melampsora larici-populina using an innovative single-spore inoculation procedure. A set of quantitative fungal traits (infection efficiency, latent period, uredinia size, mycelium quantity, sporulation rate, sporulation capacity, and spore volume) was measured on each infected leaf disk. Uninfected parts of the leaves were analyzed for their nutrient (sugars, total C and N) and defense compounds (phenolics) content. We found that M. larici-populina is more aggressive on more mature leaves as indicated by wider uredinia and a higher sporulation rate. Other traits varied independently from each other without a consistent pattern. None of the pathogen traits correlated with leaf sugar, total C, or total N content. In contrast, phenolic contents (flavonols, hydroxycinnamic acid esters, and salicinoids) were negatively correlated with uredinia size and sporulation rate. The pathogen’s fitness appeared to be more constrained by the constitutive plant defense level than limited by nutrient availability, as evident in the decrease in sporulation.

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How to cite 

Maupetit Agathe, Larbat Romain, Pernaci Michaël, Andrieux Axelle, Guinet Cécile, Boutigny Anne-Laure, Fabre Bénédicte, Frey Pascal, Halkett Fabien (2018). Defense Compounds Rather Than Nutrient Availability Shape Aggressiveness Trait Variation Along a Leaf Maturity Gradient in a Biotrophic Plant Pathogen. Frontiers in Plant Science, 9(1396), 13p. Publisher's official version : https://doi.org/10.3389/fpls.2018.01396 , Open Access version : https://archimer.ifremer.fr/doc/00652/76405/