Responses to iron oxide and zinc oxide nanoparticles in echinoderm embryos and microalgae: uptake, growth, morphology, and transcriptomic analysis

Type Article
Date 2020-11
Language English
Author(s) Genevière Anne-Marie1, Derelle Evelyne1, 2, Escande Marie-Line1, Grimsley Nigel1, Klopp Christophe3, Ménager Christine4, Michel Aude4, Moreau Hervé1
Affiliation(s) 1 : Sorbonne Université, CNRS, Biologie Intégrative des Organismes Marins, BIOM, Banyuls-sur-Mer, France
2 : Univ Brest, CNRS, IRD, Ifremer, LEMAR, Plouzane, France
3 : INRA, Plateforme Bioinformatique Toulouse, Midi Pyrenees UBIA, Castanet Tolosan, France
4 : Sorbonne Université, CNRS, PHysico-chimie des Electrolytes et Nanosystèmes InterfaciauX, PHENIX, Paris, France
Source Nanotoxicology (1743-5390) (Informa UK Limited), 2020-11 , Vol. 14 , N. 10 , P. 1342-1361
DOI 10.1080/17435390.2020.1827074
Keyword(s) Nanoparticles, toxicity, marine organisms, metal oxide, transcriptomics
Abstract

We investigated the toxicity of Iron oxide and Zinc oxide engineered nanoparticles (ENPs) on Paracentrotus lividus sea urchin embryos and three species of microalgae. Morphological responses, internalization, and potential impacts of Fe2O3 and ZnO ENPs on physiology and metabolism were assessed. Both types of ENPs affected P. lividus larval development, but ZnO ENPs had a much stronger effect. While growth of the alga Micromonas commoda was severely impaired by both ENPs, Ostreococcus tauri or Nannochloris sp. were unaffected. Transmission electron microscopy showed the internalization of ENPs in sea urchin embryonic cells while only nanoparticle interaction with external membranes was evidenced in microalgae, suggesting that marine organisms react in diverse ways to ENPs. Transcriptome-wide analysis in P. lividus and M. commoda showed that many different physiological pathways were affected, some of which were common to both species, giving insights about the mechanisms underpinning toxic responses

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Genevière Anne-Marie, Derelle Evelyne, Escande Marie-Line, Grimsley Nigel, Klopp Christophe, Ménager Christine, Michel Aude, Moreau Hervé (2020). Responses to iron oxide and zinc oxide nanoparticles in echinoderm embryos and microalgae: uptake, growth, morphology, and transcriptomic analysis. Nanotoxicology, 14(10), 1342-1361. Publisher's official version : https://doi.org/10.1080/17435390.2020.1827074 , Open Access version : https://archimer.ifremer.fr/doc/00654/76607/