Deciphering the Infectious Process of Colletotrichum lupini in Lupin through Transcriptomic and Proteomic Analysis

Type Article
Date 2020-10
Language English
Author(s) Dubrulle Guillaume1, Picot Adeline1, Madec Stephanie2, Corre Erwan3, Pawtowski Audrey1, Baroncelli Riccardo4, Zivy Michel5, Balliau Thierry5, Le Floch Gaétan1, Pensec Flora1
Affiliation(s) 1 : Laboratoire Universitaire de Biodiversité et Écologie Microbienne, ESIAB, Université de Brest, F-29280 Plouzané
2 : CNRS, IRD, Ifremer, LEMAR, Université de Brest, F-29280 Plouzané, France
3 : Station Biologique de Roscoff, FR2424 CNRS Sorbonne Université, Place Georges Teissier, 29680 Roscoff, France
4 : Instituto Hispano-Luso de Investigaciones Agrarias (CIALE), University of Salamanca, Calle del Duero 12, 37185 Villamayor (Salamanca), Spain
5 : INRAE le Moulon, Plateforme PAPPSO, ferme du Moulon, 91190 Gif-sur-Yvette, France
Source Microorganisms (2076-2607) (MDPI AG), 2020-10 , Vol. 8 , N. 10 , P. 1621 (33p.)
DOI 10.3390/microorganisms8101621
WOS© Times Cited 16
Note This article belongs to the Section Plant Microbe Interactions
Keyword(s) anthracnose disease, transcriptome, proteome, pathogenicity factors

The fungal phytopathogen Colletotrichum lupini is responsible for lupin anthracnose, resulting in significant yield losses worldwide. The molecular mechanisms underlying this infectious process are yet to be elucidated. This study proposes to evaluate C. lupini gene expression and protein synthesis during lupin infection, using, respectively, an RNAseq-based transcriptomic approach and a mass spectrometry-based proteomic approach. Patterns of differentially-expressed genes in planta were evaluated from 24 to 84 hours post-inoculation, and compared to in vitro cultures. A total of 897 differentially-expressed genes were identified from C. lupini during interaction with white lupin, of which 520 genes were predicted to have a putative function, including carbohydrate active enzyme, effector, protease or transporter-encoding genes, commonly described as pathogenicity factors for other Colletotrichum species during plant infection, and 377 hypothetical proteins. Simultaneously, a total of 304 proteins produced during the interaction were identified and quantified by mass spectrometry. Taken together, the results highlight that the dynamics of symptoms, gene expression and protein synthesis shared similarities to those of hemibiotrophic pathogens. In addition, a few genes with unknown or poorly-described functions were found to be specifically associated with the early or late stages of infection, suggesting that they may be of importance for pathogenicity. This study, conducted for the first time on a species belonging to the Colletotrichum acutatum species complex, presents an opportunity to deepen functional analyses of the genes involved in the pathogenicity of Colletotrichum spp. during the onset of plant infection.

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Dubrulle Guillaume, Picot Adeline, Madec Stephanie, Corre Erwan, Pawtowski Audrey, Baroncelli Riccardo, Zivy Michel, Balliau Thierry, Le Floch Gaétan, Pensec Flora (2020). Deciphering the Infectious Process of Colletotrichum lupini in Lupin through Transcriptomic and Proteomic Analysis. Microorganisms, 8(10), 1621 (33p.). Publisher's official version : , Open Access version :