High temperature induces transcriptomic changes in Crassostrea gigas that hinder progress of ostreid herpesvirus (OsHV-1) and promote survival

Type Article
Date 2020-10
Language English
Author(s) Delisle Lizenn1, 2, Pauletto Marianna3, Vidal-Dupiol JeremieORCID4, Petton Bruno1, Bargelloni Luca3, Montagnani Caroline4, Pernet FabriceORCID1, Corporeau Charlotte1, Fleury ElodieORCID1
Affiliation(s) 1 : Ifremer, Université de Brest, CNRS, IRD, LEMAR, F-29280 Plouzané, France
2 : Cawthron Institute, 98 Halifax Street East, Private Bag 2, Nelson 7042, New Zealand
3 : Department of Comparative Biomedicine and Food Science, University of Padova, Viale dell'Università 16, 35020 Legnaro, Padova, Italy
4 : IHPE, Université de Montpellier, CNRS, Ifremer, Université de Perpignan, Via Domitia, F-34095 Montpellier, France
Source Journal Of Experimental Biology (0022-0949) (The Company of Biologists), 2020-10 , Vol. 223 , N. 20 , P. jeb226233 (11p.)
DOI 10.1242/jeb.226233
WOS© Times Cited 3
Keyword(s) Anti-viral molecular pathway, Host-pathogen interaction, Marine disease, OsHV-1, Resistance, Temperature
Abstract

Of all environmental factors, seawater temperature plays a decisive role in triggering marine diseases. Like fever in vertebrates, high seawater temperature could modulate the host response to pathogens in ectothermic animals. In France, massive mortality of Pacific oysters, Crassostrea gigas, caused by the ostreid herpesvirus 1 (OsHV-1) is markedly reduced when temperatures exceed 24°C in the field. In the present study we assess how high temperature influences the host response to the pathogen by comparing transcriptomes (RNA sequencing) during the course of experimental infection at 21°C (reference) and 29°C. We show that high temperature induced host physiological processes that are unfavorable to the viral infection. Temperature influenced the expression of transcripts related to the immune process and increased the transcription of genes related to the apoptotic process, synaptic signaling and protein processes at 29°C. Concomitantly, the expression of genes associated with catabolism, metabolite transport, macromolecule synthesis and cell growth remained low from the first stage of infection at 29°C. Moreover, viral entry into the host might have been limited at 29°C by changes in extracellular matrix composition and protein abundance. Overall, these results provide new insights into how environmental factors modulate host–pathogen interactions.

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Delisle Lizenn, Pauletto Marianna, Vidal-Dupiol Jeremie, Petton Bruno, Bargelloni Luca, Montagnani Caroline, Pernet Fabrice, Corporeau Charlotte, Fleury Elodie (2020). High temperature induces transcriptomic changes in Crassostrea gigas that hinder progress of ostreid herpesvirus (OsHV-1) and promote survival. Journal Of Experimental Biology, 223(20), jeb226233 (11p.). Publisher's official version : https://doi.org/10.1242/jeb.226233 , Open Access version : https://archimer.ifremer.fr/doc/00656/76806/