An improved whole life cycle culture protocol for the hydrozoan genetic model Clytia hemisphaerica

Type Article
Date 2020-11
Language English
Author(s) Lechable Marion1, Jan Anne-Cecile1, 2, Duchene Axel2, Uveira Julie1, Weissbourd Brandon3, Gissat Loann2, Collet Sophie2, Gilletta Laurent1, Chevalier Sandra1, Leclère Lucas1, Peron Sophie1, Barreau Carine1, Lasbleiz Régis2, Houliston Evelyn1, Momose Tsuyoshi1
Affiliation(s) 1 : Sorbonne Universités, CNRS, Institut de la Mer de Villefranche, Laboratoire de Biologie du Développement de Villefranche-sur-Mer (LBDV), 06230 Villefranche-sur-Mer, France
2 : Sorbonne Universités, Institut de la mer de Villefranche, FR3761, Service Aquariologie, 06230 Villefranche-sur-Mer, France
3 : California Institute of Technology, Division of Biology, and the Howard Hughes Medical Institute, 1200 E California Blvd, Pasadena CA 91125, USA
Source Biology Open (2046-6390) (The Company of Biologists), 2020-11 , Vol. 9 , N. 11 , P. bio051268 (13p.)
DOI 10.1242/bio.051268
WOS© Times Cited 1
Keyword(s) Animal culture, Cnidarian, Developmental biology, Genetics, Jellyfish
Abstract

The jellyfish species Clytia hemisphaerica (Cnidaria, Hydrozoa) has emerged as a new experimental model animal in the last decade. Favorable characteristics include a fully transparent body suitable for microscopy, daily gamete production and a relatively short life cycle. Furthermore, whole genome sequence assembly and efficient gene editing techniques using CRISPR/Cas9 have opened new possibilities for genetic studies. The quasi-immortal vegetatively-growing polyp colony stage provides a practical means to maintain mutant strains. In the context of developing Clytia as a genetic model, we report here an improved whole life cycle culture method including an aquarium tank system designed for culture of the tiny jellyfish form. We have compared different feeding regimes using Artemia larvae as food and demonstrate that the stage-dependent feeding control is the key for rapid and reliable medusa and polyp rearing. Metamorphosis of the planula larvae into a polyp colony can be induced efficiently using a new synthetic peptide. The optimized procedures detailed here make it practical to generate genetically modified Clytia strains and to maintain their whole life cycle in the laboratory.

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Lechable Marion, Jan Anne-Cecile, Duchene Axel, Uveira Julie, Weissbourd Brandon, Gissat Loann, Collet Sophie, Gilletta Laurent, Chevalier Sandra, Leclère Lucas, Peron Sophie, Barreau Carine, Lasbleiz Régis, Houliston Evelyn, Momose Tsuyoshi (2020). An improved whole life cycle culture protocol for the hydrozoan genetic model Clytia hemisphaerica. Biology Open, 9(11), bio051268 (13p.). Publisher's official version : https://doi.org/10.1242/bio.051268 , Open Access version : https://archimer.ifremer.fr/doc/00656/76820/