A rapid diagnostic multiplex PCR approach for xenomonitoring of human and animal schistosomiasis in a 'One Health' context

Type Article
Date 2019-11
Language English
Author(s) Schols RubenORCID1, Carolus HansORCID1, Hammoud Cyril2, 3, Mulero StephenORCID4, Mudavanhu AspireORCID5, Huyse Tine1, 2
Affiliation(s) 1 : Univ Leuven, Lab Biodivers & Evolutionary Genom, B-3000 Leuven, Belgium.
2 : Royal Museum Cent Africa, Dept Biol, Leuvensesteenweg 13, B-3080 Tervuren, Belgium.
3 : Univ Ghent, Limnol Res Unit, KL Ledeganckstr 35, B-9000 Ghent, Belgium.
4 : Univ Perpignan, CNRS, Lab Host Pathogen Environm Interact, IHPE UMR 5244, Via Domitia, F-66100 Perpignan, France.
5 : Univ Zimbabwe, Dept Biol Sci, Harare, Zimbabwe.
Source Transactions Of The Royal Society Of Tropical Medicine And Hygiene (0035-9203) (Oxford Univ Press), 2019-11 , Vol. 113 , N. 11 , P. 722-729
DOI 10.1093/trstmh/trz067
WOS© Times Cited 20
Keyword(s) gastropod-borne disease, One Health, rapid diagnostic multiplex PCR, Schistosoma, transmission monitoring, Trematoda
Abstract Studying the epidemiology of schistosomiasis-the most prevalent gastropod-borne human disease and an economic burden for the livestock industry-relies on adequate monitoring tools. Here we describe a molecular assay for detecting human and animal African schistosome species in their planorbid gastropod host (xenomonitoring) using a two-step approach. First, schistosome infections are detected and discriminated from other trematode infections using a multiplex polymerase chain reaction (PCR) that includes a trematode-specific marker (in 18S rDNA), a Schistosoma genus-specific marker (in internal transcribed spacer 2 [ITS2]) and a general gastropod marker (in 18S rDNA) as an internal control. Upon Schistosoma sp. detection, a second multiplex PCR is performed to discriminate among Schistosoma haematobium, Schistosoma mansoni, Schistosoma mattheei and Schistosoma bovis/Schistosoma curassoni/Schistosoma guineensis using markers of differential lengths in the cytochrome c oxidase subunit 1 (COX1) gene. The specificity of these assays was validated with adult worms, naturally infected gastropods and human urine and stool samples. Sensitivity was tested on experimentally infected snail specimens that were sacrificed 10 and 40 days post-infection in order to mimic a natural prepatent and mature infection, respectively. The assay provides a diagnostic tool to support the xenomonitoring of planorbid gastropods for trematode infections in a One Health context, with a focus on the transmission monitoring of schistosomiasis.
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Schols Ruben, Carolus Hans, Hammoud Cyril, Mulero Stephen, Mudavanhu Aspire, Huyse Tine (2019). A rapid diagnostic multiplex PCR approach for xenomonitoring of human and animal schistosomiasis in a 'One Health' context. Transactions Of The Royal Society Of Tropical Medicine And Hygiene, 113(11), 722-729. Publisher's official version : https://doi.org/10.1093/trstmh/trz067 , Open Access version : https://archimer.ifremer.fr/doc/00659/77115/