Alterins Produced by Oyster-Associated Pseudoalteromonas are Antibacterial Cyclolipopeptides with LPS-Binding Activity

Type Article
Date 2020-12
Language English
Author(s) Desriac Florie1, El Harras Abderrafek2, Simon Matthieu2, Bondon Arnaud2, Brillet Benjamin1, Le Chevalier Patrick1, Pugnière Martine3, Got Patrice4, Destoumieux-Garzón Delphine5, Fleury Yannick1
Affiliation(s) 1 : Laboratoire de Biotechnologie et Chimie Marine, EA3884, Université de Bretagne Occidentale, Université Bretagne Sud, 29334 Quimper, France
2 : Institut des Sciences Chimiques de Rennes-CNRS-UMR 6226, Université Rennes, 35043 Rennes, France
3 : IRCM, Institut de Recherche en Cancérologie de Montpellier, INSERM U1194, Université de Montpellier, Institut régional du Cancer de Montpellier, 34298 Montpellier, France
4 : MARBEC Université de Montpellier, CNRS, IRD, Place Eugène Bataillon CC 093, Place Eugène Bataillon, CEDEX 5, 34095 Montpellier, France
5 : Interactions Hôtes-Pathogènes-Environnements, Université de Montpellier, CNRS, Ifremer, Université Perpignan Via Domitia, 34095 Montpellier, France
Source Marine Drugs (1660-3397) (MDPI AG), 2020-12 , Vol. 18 , N. 12 , P. 630 (16p.)
DOI 10.3390/md18120630
Note This article belongs to the Special Issue Marine Antibiotics 2020
Keyword(s) Pseudoalteromonas, cyclolipopeptides, alterin, antibiotic
Abstract

Discovery after discovery, host-associated microbiota reveal a growing list of positive effects on host homeostasis by contributing to host nutrition, improving hosts’ immune systems and protecting hosts against pathogens. In that context, a collection of oyster associated bacteria producing antibacterial compounds have been established to evaluate their role in non-host-derived immunity. Here, we described alterins; potent anti-Gram negative compounds produced by Pseudoalteromonas hCg-6 and hCg-42 isolated from different healthy oyster hemolymph. The strains hCg-6 and hCg-42 produce a set of at least seven antibacterial compounds, ranging from 926 to 982 Da structurally characterized as cyclolipopeptides (CLPs). Alterins share the same cationic heptapeptidic cycle connected via an amido bond to different hydrophobic hydrocarbon tails. Their MICs disclosed a potent antibacterial activity directed against Gram-negative bacteria including oyster and human pathogens that may confer a beneficial defense mechanism to the host but also represents an untapped source of new antibiotics. The alterins’ mechanisms of action have been deciphered: after binding to lipopolysaccharides (LPS), alterins provoke a membrane depolarization and permeabilization leading to bacterial lysis. As hCg-6 and hCg-42 produced a set of natural derivatives, the structure/activity relationship linked to the carbon tail is clarified. We showed that the hydrocarbon tail determines the LPS-binding properties of alterins and consequently their antibacterial activities. Its length and saturation seem to play a major role in this interaction

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Desriac Florie, El Harras Abderrafek, Simon Matthieu, Bondon Arnaud, Brillet Benjamin, Le Chevalier Patrick, Pugnière Martine, Got Patrice, Destoumieux-Garzón Delphine, Fleury Yannick (2020). Alterins Produced by Oyster-Associated Pseudoalteromonas are Antibacterial Cyclolipopeptides with LPS-Binding Activity. Marine Drugs, 18(12), 630 (16p.). Publisher's official version : https://doi.org/10.3390/md18120630 , Open Access version : https://archimer.ifremer.fr/doc/00663/77511/