Identification and expression profile analysis of odorant binding protein and chemosensory protein genes in Bemisia tabaci MED by head transcriptome

Type Article
Date 2017-02
Language English
Author(s) Wang Ran1, Li Fengqi1, Zhang Wei1, Zhang Xiaoman1, Qu Cheng1, Tetreau GuillaumeORCID2, Sun Lujuan3, Luo Chen1, Zhou Jingjiang4
Affiliation(s) 1 : Beijing Acad Agr & Forestry Sci, Inst Plant & Environm Protect, Beijing, Peoples R China.
2 : Univ Perpignan, Lab Interact Hotes Pathogenes Environm, Perpignan, France.
3 : Chinese Acad Agr Sci, Agr Informat Inst, Beijing, Peoples R China.
4 : Rothamsted Res, Dept Biol Chem & Crop Protect, Harpenden, Herts, England.
Source Plos One (1932-6203) (Public Library Science), 2017-02 , Vol. 12 , N. 2 , P. e0171739 (19p.)
DOI 10.1371/journal.pone.0171739
WOS© Times Cited 29
Abstract

Odorant binding proteins (OBPs) and chemosensory proteins (CSPs) of arthropods are thought to be involved in chemical recognition which regulates pivotal behaviors including host choice, copulation and reproduction. In insects, OBPs and CSPs located mainly in the antenna but they have not been systematically characterized yet in Bemisia tabaci which is a cryptic species complex and could damage more than 600 plant species. In this study, among the 106,893 transcripts in the head assembly, 8 OBPs and 13 CSPs were identified in B. tabaci MED based on head transcriptomes of adults. Phylogenetic analyses were conducted to investigate the relationships of B. tabaci OBPs and CSPs with those from several other important Hemipteran species, and the motif-patterns between Hemiptera OBPs and CSPs were also compared by MEME. The expression profiles of the OBP and CSP genes in different tissues of B. tabaci MED adults were analyzed by real-time qPCR. Seven out of the 8 OBPs found in B. tabaci MED were highly expressed in the head. Conversely, only 4 CSPs were enriched in the head, while the other nine CSPs were specifically expressed in other tissues. Our findings pave the way for future research on chemical recognition of B. tabaci at the molecular level.

Full Text
File Pages Size Access
Publisher's official version 19 2 MB Open access
S1 Table. PCR primers. 2 84 KB Open access
S2 Table. RT-qPCR primers. 2 87 KB Open access
S1 File. Sequences for phylogenetic analysis. 14 104 KB Open access
26 194 KB Access on demand
Top of the page

How to cite 

Wang Ran, Li Fengqi, Zhang Wei, Zhang Xiaoman, Qu Cheng, Tetreau Guillaume, Sun Lujuan, Luo Chen, Zhou Jingjiang (2017). Identification and expression profile analysis of odorant binding protein and chemosensory protein genes in Bemisia tabaci MED by head transcriptome. Plos One, 12(2), e0171739 (19p.). Publisher's official version : https://doi.org/10.1371/journal.pone.0171739 , Open Access version : https://archimer.ifremer.fr/doc/00663/77537/