In vitro evaluation of polymeric nanoparticles with a fluorine core for drug delivery triggered by focused ultrasound

Type Article
Date 2021-04
Language English
Author(s) Somaglino LucieORCID1, Mousnier L2, Giron A1, Urbach W1, 3, Tsapis N2, Taulier N1
Affiliation(s) 1 : Sorbonne Université, CNRS, INSERM, Laboratoire d’Imagerie Biomédicale, LIB, F-75006 Paris, France
2 : Université Paris-Saclay, CNRS, Institut Galien Paris Saclay, 92296, Châtenay-Malabry, France
3 : Laboratoire de Physique de l’École Normale Supérieure, ENS, Université PSL, CNRS, Sorbonne Université, Université de Paris, F-75005 Paris, France
Source Colloids And Surfaces B-biointerfaces (0927-7765) (Elsevier), 2021-04 , Vol. 200 , P. 111561 (8p.)
DOI 10.1016/j.colsurfb.2021.111561
WOS© Times Cited 8
Keyword(s) Ultrasound, Drug delivery, Nanoparticles, PFOB, Cavitation

Polymeric nanoparticles are being intensively investigated as drug carriers. Their efficiency could be enhanced if the drug release can be triggered using an external stimulus such as ultrasound. This approach is possible using current commercial apparatus that combine focused ultrasound with MRI to perform ultrasonic surgery. In this approach, nanoparticles made of a perfluoro-octyl bromide core and a thick polymeric (PLGA-PEG) shell may represent suitable drug carriers. Indeed, their perfluorocarbon core are detectable by 19F MRI, while their polymeric shell can encapsulate drugs. However, their applicability in ultrasound-triggered drug delivery remains to be proven. To do so, we used Nile red as a model drug and we measured its release from the polymeric shell by spectrofluorometry. In the absence of ultrasound, only a small amount of Nile red release was measured (<5%). Insonations were performed in a controlled environment using a 1.1 MHz transducer emitting tone bursts for a few minutes, whereas a focused broadband hydrophone was used to detect the occurrence of cavitation. In the absence of detectable inertial cavitation, less than 5% of Nile red was released. In the presence of detectable inertial cavitation, Nile red release was ranging from 10 to 100%, depending of the duty cycle, acoustic pressure, and tank temperature (25 or 37°C). Highest releases were obtained only for duty cycles of 25% at 37°C and 50% at 25°C and for a peak-to-peak acoustic pressure above 12.7 MPa. Electron microscopy and light scattering measurements showed a slight modification in the nanoparticle morphology only at high release contents. The occurrence of strong inertial cavitation is thus a prerequisite to induce drug release for these nanoparticles. Since strong inertial cavitation can lead to many unwanted biological effects, these nanoparticles may not be suitable for a therapeutic application using ultrasound-triggered drug delivery.

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