The Digestive Function of Pseudoplatystoma punctifer Early Juveniles Is Differentially Modulated by Dietary Protein, Lipid and Carbohydrate Content and Their Ratios

Type Article
Date 2021-02
Language English
Author(s) Castro-Ruiz DianaORCID1, Andree Karl B.2, Solovyev Mikhail M.ORCID3, 4, Fernández-Méndez Christian5, García-Dávila Carmen1, Cahu Chantal6, Gisbert EnricORCID2, Darias Maria J.ORCID7
Affiliation(s) 1 : Laboratorio de Biología y Genética Molecular (LBGM), Dirección de Investigación en Ecosistemas Acuáticos Amazónicos (AQUAREC), Instituto de Investigaciones de la Amazonía Peruana (IIAP), Carretera Iquitos-Nauta km 4.5, Iquitos, Peru
2 : Aquaculture Program, Centre de Sant Carles de la Ràpita (IRTA-SCR), Institut de Recerca i Tecnologìa Agroalimentaries (IRTA), Crta. Poble Nou km 5.5, 43540 Sant Carles de la Ràpita, Spain
3 : Institute of Systematics and Ecology of Animals Siberian Branch, Russian Academy of Sciences, 11 Frunze St., 630091 Novosibirsk, Russia
4 : Tomsk State University, 36 Lenin Ave., 634050 Tomsk, Russia
5 : Laboratorio de Bromatología, Dirección de Investigación en Ecosistemas Acuáticos Amazónicos (AQUAREC), Instituto de Investigaciones de la Amazonía Peruana (IIAP), Carretera Iquitos-Nauta km. 4.5, Iquitos, Peru
6 : LEMAR, Univ Bretagne, CNRS, Ifremer, IRD, 29280 Plouzané, France
7 : MARBEC, Univ Montpellier, CNRS, Ifremer, IRD, Montpellier, France
Source Animals (2076-2615) (MDPI AG), 2021-02 , Vol. 11 , N. 2 , P. 369 (27p.)
DOI 10.3390/ani11020369
WOS© Times Cited 3
Keyword(s) neotropical fish, aquaculture, digestive enzymes, gene expression, nutrition, juveniles, histology, development, macronutrients, diet
Abstract

Pseudoplatystoma punctifer is an Amazonian catfish highly appreciated for its high flesh quality, size, and commercial value. Its aquaculture is pursued to satisfy the demands of an increasing population in the region. However, knowledge of the nutritional needs during the early life stages is necessary for improving growth and reducing the incidence of cannibalism, factors that limit the success of its commercial farming. This study aimed at evaluating the influence of four diets containing different protein and lipid levels (30:15, 30:10, 45:15, or 45:10 in %) in the digestive physiology and performance of early juveniles. The results showed that the dietary protein:lipid as well as carbohydrate levels and ratios influenced differently the whole-body proximate composition, the digestive physiology and development, and hence growth and survival. The 45:15 diet promoted the best growth, survival, and the most rapid development of the digestive system, as shown at histological (higher number of hepatocytes, goblet cells in the anterior intestine and enterocytes in all intestinal portions, and longer folds in the posterior intestine), molecular (highest amylase, lipoprotein lipase, phospholipase, trypsinogen, and pepsinogen gene expression), and biochemical (highest lipase and pepsin activities and higher alkaline phosphatase:leucine alanine peptidase activity ratio) levels. Lipids were favored over carbohydrates as source of energy, with lipids promoting a protein-sparing effect at adequate energy:protein ratio. Carbohydrate content higher than 25% was excessive for this species, leading to unbalanced lipid metabolism and fat deposition in the liver.

Full Text
File Pages Size Access
Publisher's official version 27 2 MB Open access
Top of the page

How to cite 

Castro-Ruiz Diana, Andree Karl B., Solovyev Mikhail M., Fernández-Méndez Christian, García-Dávila Carmen, Cahu Chantal, Gisbert Enric, Darias Maria J. (2021). The Digestive Function of Pseudoplatystoma punctifer Early Juveniles Is Differentially Modulated by Dietary Protein, Lipid and Carbohydrate Content and Their Ratios. Animals, 11(2), 369 (27p.). Publisher's official version : https://doi.org/10.3390/ani11020369 , Open Access version : https://archimer.ifremer.fr/doc/00678/79019/