The last time in Earth's history when high latitudes were warmer than during pre-industrial times was the last interglacial period (LIG, 129–116 ka BP). Since the LIG is the most recent and best documented interglacial, it can provide insights into climate processes in a warmer world. However, some key features of the LIG are not well constrained, notably the oceanic circulation and the global carbon cycle. Here, we use a new database of LIG benthic δ13C to investigate these two aspects. We find that the oceanic mean δ13C was  ∼  0.2 ‰ lower during the LIG (here defined as 125–120 ka BP) when compared to the Holocene (7–2 ka BP). A lower terrestrial carbon content at the LIG than during the Holocene could have led to both lower oceanic δ13C and atmospheric δ13CO2 as observed in paleo-records. However, given the multi-millennial timescale, the lower oceanic δ13C most likely reflects a long-term imbalance between weathering and burial of carbon. The δ13C distribution in the Atlantic Ocean suggests no significant difference in the latitudinal and depth extent of North Atlantic Deep Water (NADW) between the LIG and the Holocene. Furthermore, the data suggest that the multi-millennial mean NADW transport was similar between these two time periods.