Deeper insight into Gambierdiscus polynesiensis toxin production relies on specific optimization of High-Performance Liquid Chromatography High Resolution Mass Spectrometry

Type Article
Date 2021-09
Language English
Author(s) Yon ThomasORCID1, Sibat ManoellaORCID1, Réveillon DamienORCID1, Bertrand Samuel2, 3, Chinain MireilleORCID4, Hess PhilippORCID1
Affiliation(s) 1 : IFREMER, DYNECO, Laboratoire Phycotoxines, F-44000 Nantes, France
2 : Université de Nantes, MMS, EA 2160, Nantes, France
3 : ThalassOMICS Metabolomics Facility, Plateforme Corsaire, Biogenouest, Nantes, France
4 : Institut Louis Malardé, UMR 241 EIO, 98713 Papeete, Tahiti, French Polynesia
Source Talanta (0039-9140) (Elsevier BV), 2021-09 , Vol. 232 , P. 122400 (11p.)
DOI 10.1016/j.talanta.2021.122400
WOS© Times Cited 4
Keyword(s) Mass spectrometry, Gambierdiscus polynesiensis, Ciguatoxins, Gambierones, Fractional factorial design
Abstract

Ciguatera food poisoning affects consumer health and fisheries’ economies worldwide in tropical zones, and specifically in the Pacific area. The wide variety of ciguatoxins bio-accumulated in fish or shellfish responsible for this neurological illness are produced by marine dinoflagellates of the genus Gambierdiscus and bio-transformed trough the food web. The evaluation of the contents of ciguatoxins in strains of Gambierdiscus relies on the availability of standards and on the development of sensitive and specific tools to detect them. There is a need for sensitive methods for the analysis of pacific ciguatoxins with high resolution mass spectrometry to ensure unequivocal identification of all congeners. We have applied a fractional factorial design of experiment 2ˆ8-3 for the screening of the significance of eight parameters potentially influencing ionization and ion transmission and their interactions to evaluate the behavior of sodium adducts, protonated molecules and first water losses of CTX4A/B, CTX3B/C, 2-OH-CTX3C and 44-methylgambierone on a Q-TOF equipment. The four parameters that allowed to significantly increase the peak areas of ciguatoxins and gambierones (up to a factor ten) were the capillary voltage, the sheath gas temperature, the ion funnel low pressure voltage and the ion funnel exit voltage. The optimized method was applied to revisit the toxin profile of G. polynesiensis (strain TB92) with a confirmation of the presence of M-seco-CTX4A only putatively reported so far and the detection of an isomer of CTX4A. The improvement in toxin detection also allowed to obtain informative high resolution targeted MS/MS spectra revealing high similarity in fragmentation patterns between putative isomer (4) of CTX3C, 2-OH-CTX3C and CTX3B on one side and between CTX4A, M-seco-CTX4A and the putative isomer on the other side suggesting a relation of constitutional isomerism between them for both isomers.

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Yon Thomas, Sibat Manoella, Réveillon Damien, Bertrand Samuel, Chinain Mireille, Hess Philipp (2021). Deeper insight into Gambierdiscus polynesiensis toxin production relies on specific optimization of High-Performance Liquid Chromatography High Resolution Mass Spectrometry. Talanta, 232, 122400 (11p.). Publisher's official version : https://doi.org/10.1016/j.talanta.2021.122400 , Open Access version : https://archimer.ifremer.fr/doc/00689/80147/