Study differentiating fish oocyte developmental stages using bioimpedance spectroscopy

Type Article
Date 2022-01
Language English
Author(s) Detrez EloïseORCID1, 2, Kerzérho VincentORCID1, Belhaj Mohamed-Moez1, Vergnet Alain2, de Verdal Hugues3, 4, Rouyer TristanORCID2, Bonhommeau SylvainORCID5, Lamlih AchrafORCID1, Julien Mohan1, Ali Fathi Ben1, Renovell MichelORCID1, Bernard Serge1, Soulier Fabien1
Affiliation(s) 1 : LIRMM, Univ Montpellier, CNRS, Montpellier, France
2 : IFREMER, Univ Montpellier, MARBEC, Sète, France
3 : CIRAD, UMR ISEM, Montpellier, France
4 : ISEM, Univ Montpellier, CNRS, EPHE, IRD, Montpellier, France
5 : IFREMER/DOI, Le Port, France
Source Aquaculture (0044-8486) (Elsevier BV), 2022-01 , Vol. 547 , P. 737396 (7p.)
DOI 10.1016/j.aquaculture.2021.737396
WOS© Times Cited 2
Keyword(s) Bioimpedance, Spectroscopy, Dicentrarchus labrax, Oocyte developmental stages

Fish oocyte development monitoring is a mandatory operation when studying breeding in captivity as well as in the wild. In aquaculture, it is required to determine the best fertilization time. In the wild, it helps to detect spawning grounds, the distribution of fish species and the interaction between animals and their environment. In both settings, the conventional technique for developmental stage identification consists of handling the fish for oocyte sampling and sample observation using a binocular zoom head. Such an operation is difficult for the operator as well as for the fish. There is a need for repeated anesthesia and oocyte sampling, and it relies on operator expertise to identify the developmental stage. In this context, this publication proposes, for the first time, to study the potential of bioimpedance measurement as an alternative in fish breeding studies. We have set up an experiment combining the in vitro bioimpedance measurement of sampled oocytes with the conventional estimation technique for 69 sampled collected on farmed European sea bass. The statistical analysis has demonstrated that three of the four main developmental stages can be identified using bioimpedance measurement. The integrability of the bioimpedance technique with its implantable sensor makes this potential alternative approach very promising. Thanks to this sensor it could be possible, for the first time ever, to monitor in vivo the oocyte developmental stage in captivity as well as in the wild.

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Detrez Eloïse, Kerzérho Vincent, Belhaj Mohamed-Moez, Vergnet Alain, de Verdal Hugues, Rouyer Tristan, Bonhommeau Sylvain, Lamlih Achraf, Julien Mohan, Ali Fathi Ben, Renovell Michel, Bernard Serge, Soulier Fabien (2022). Study differentiating fish oocyte developmental stages using bioimpedance spectroscopy. Aquaculture, 547, 737396 (7p.). Publisher's official version : , Open Access version :