The impact of the multichannel quorum sensing systems of Vibrio tasmaniensis and Vibrio crassostreae on virulence towards blue mussel (Mytilus edulis) larvae

Type Article
Date 2022-01
Language English
Author(s) Islam Shikder Saiful1, 2, Zhang Shanshan3, Eggermont Mieke3, Bruto Maxime4, Le Roux Frederique4, Defoirdt Tom3
Affiliation(s) 1 : Fisheries and Marine Resource Technology Discipline, Khulna University, Khulna, Bangladesh
2 : Institute for Marine and Antarctic Studies, University of Tasmania, Launceston, Australia
3 : Center for Microbial Ecology and Technology (CMET), Ghent University, Gent, Belgium
4 : Station Biologique de Roscoff, Roscoff, France
Source Aquaculture (0044-8486) (Elsevier), 2022-01 , Vol. 547 , P. 737414 (6p.)
DOI 10.1016/j.aquaculture.2021.737414
WOS© Times Cited 5
Keyword(s) Bivalve, Splendidus clade, Larviculture, Immersion challenge, Quorum sensing
Abstract

Mussel culture in Europe is currently suffering from mass mortality events with mortality rates up to 100%. Vibrios belonging to the Splendidus clade have recently been implicated in these events. In this study, we demonstrate that V. tasmaniensis LGP32 and V. crassostreae J2-9, two model pathogens of oysters, are also pathogenic to blue mussel larvae in an immersion challenge test. After five days, LGP32 and J2-9 had killed 93 and 73% of challenged mussel larvae, respectively. Because quorum sensing, bacterial cell-to-cell communication with small signal molecules, has been demonstrated to control the virulence of various vibrios, we further investigated whether it had an impact on the virulence of V. tasmaniensis and V. crassostreae. We identified the components of a multichannel quorum sensing system (as also found in many other vibrios) in the genomes of both species. Knock out of selected components of this pathway revealed that in general quorum sensing in V. tasmaniensis LGP32 and V. crassostreae J2-9 has no impact on motility, protease activity, biofilm formation and virulence towards mussel larvae. Finally, the quorum sensing inhibitor cinnamaldehyde did not protect mussel larvae from these pathogens. Together, these data indicate that the multichannel quorum sensing systems of V. tasmaniensis LGP32 and V. crassostreae J2-9 have no impact on virulence of the bacteria towards blue mussel larvae. Hence, quorum sensing controlling virulence is not a general feature in vibrios as it has different outcomes on virulence in different species.

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Islam Shikder Saiful, Zhang Shanshan, Eggermont Mieke, Bruto Maxime, Le Roux Frederique, Defoirdt Tom (2022). The impact of the multichannel quorum sensing systems of Vibrio tasmaniensis and Vibrio crassostreae on virulence towards blue mussel (Mytilus edulis) larvae. Aquaculture, 547, 737414 (6p.). Publisher's official version : https://doi.org/10.1016/j.aquaculture.2021.737414 , Open Access version : https://archimer.ifremer.fr/doc/00744/85584/