Microgels based on Infernan, a glycosaminoglycan-mimetic bacterial exopolysaccharide, as BMP-2 delivery systems

Type Article
Date 2022-05
Language English
Author(s) Gélébart Perrine1, Cuenot Stéphane2, Sinquin CorinneORCID1, Halgand Boris3, Sourice Sophie3, 4, Le Visage Catherine3, 4, Guicheux Jérôme3, 4, 5, Colliec-Jouault SylviaORCID1, Zykwinska AgataORCID1
Affiliation(s) 1 : Ifremer, MASAE, 44300 Nantes, France
2 : Nantes Université, CNRS, Institut des Matériaux Jean Rouxel, IMN, Nantes, France
3 : Inserm, UMR 1229, RMeS, Regenerative Medicine and Skeleton, Université de Nantes, ONIRIS, F-44042 Nantes, France
4 : UFR Odontologie, Université de Nantes, Nantes, France
5 : CHU Nantes, PHU4 OTONN, Nantes, France
Source Carbohydrate Polymers (0144-8617) (Elsevier BV), 2022-05 , Vol. 284 , P. 119191 (11p.)
DOI 10.1016/j.carbpol.2022.119191
WOS© Times Cited 6
Keyword(s) Heparin-mimetic, Capillary microfluidics, Release kinetics, BMP-2 bioactivity, Osteogenic potential, AFM imaging
Abstract

Bone Morphogenetic Protein (BMP-2) is an osteoinductive growth factor clinically used for bone regeneration. Tuneable sustained strategies for BMP-2 delivery are intensely developed to avoid severe complications related to supraphysiological doses applied. To address this issue, we investigated the ability of the bacterial exopolysaccharide (EPS) called Infernan produced by the deep-sea hydrothermal vent bacterium Alteromonas infernus, exhibiting both glycosaminoglycan-mimetic and physical gelling properties, to efficiently bind and release the bioactive BMP-2. Two delivery systems were designed based on BMP-2 retention in either single or complex EPS-based microgels, both manufactured using a microfluidic approach. BMP-2 release kinetics were highly influenced by the ionic strength, affecting both microgel stability and growth factor/EPS binding, appearing essential for BMP-2 bioactivity. The osteogenic activity of human bone-marrow derived mesenchymal stem cells studied in vitro emphasized that Infernan microgels constitute a promising platform for BMP-2 delivery for further in vivo bone repair.

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Gélébart Perrine, Cuenot Stéphane, Sinquin Corinne, Halgand Boris, Sourice Sophie, Le Visage Catherine, Guicheux Jérôme, Colliec-Jouault Sylvia, Zykwinska Agata (2022). Microgels based on Infernan, a glycosaminoglycan-mimetic bacterial exopolysaccharide, as BMP-2 delivery systems. Carbohydrate Polymers, 284, 119191 (11p.). Publisher's official version : https://doi.org/10.1016/j.carbpol.2022.119191 , Open Access version : https://archimer.ifremer.fr/doc/00750/86163/