First insights on the diversity of the genus Ostreopsis (Dinophyceae, Gonyaulacales) in Guadeloupe Island, with emphasis on the phylogenetic position of O. heptagona

Type Article
Date 2022-04
Language English
Author(s) Boisnoir Aurelie1, Bilien GwenaelORCID2, Lemée Rodolphe3, Chomérat NicolasORCID2
Affiliation(s) 1 : Ifremer, BIODIVENV, F-97231 Le Robert, Martinique, France
2 : Ifremer, LITTORAL, F-29900 Concarneau, France
3 : Sorbonne Université, CNRS, Laboratoire d’Océanographie de Villefranche, Villefranche-sur-Mer, France
Source European Journal Of Protistology (0932-4739) (Elsevier BV), 2022-04 , Vol. 83 , P. 125875 (21p.)
DOI 10.1016/j.ejop.2022.125875
WOS© Times Cited 4
Keyword(s) Amoebophrya, Concatenated phylogeny, Morpho-molecular taxonomy, Ostreopsis cf, ovata, Ostreopsis lenticularis, Ostreopsis siamensis, Ribosomal operon

The present study aims to identify epiphytic Ostreopsis cells collected in Guadeloupe between 2017 and 2018 using a morpho-molecular approach. This method combined microscopical observations of wild specimens (light and scanning electron microscopy) with a phylogenetic analysis inferred from concatenated sequences of ribosomal operon (SSU + ITS + LSU) of Ostreopsidoideae. Four distinct morphotypes were identified in our samples and studied by SEM. Molecular data obtained from single-cell PCR for the four morphotypes were consistent with observations and confirmed the presence of three Ostreopsis species resolved in well characterized genotypes (O. cf. ovata, O. lenticularis and O. siamensis) and an unidentified clade. Detailed morphological characters including sulcal plates confirmed the identification of the last morphotype as O. heptagona D.R.Norris, J.W.Bomber & Balech, which forms a new basal clade in the genus, not previously reported. Observations highlighted overlapping sizes for O. lenticularis, O. siamensis and O. heptagona. Direct sequencing of PCR products obtained for some O. lenticularis and O. heptagona collected at one site revealed unexpectedly the presence of the parasitoid dinoflagellate Amoebophrya. Some Ostreopsis cells were found partially emptied and exhibiting a compact mass. Further analyses are needed to understand the ecological role of Amoebophrya on blooms of epiphytic Ostreopsis species.

Full Text
File Pages Size Access
Author's final draft 40 2 MB Open access
21 5 MB Access on demand
242 KB Access on demand
1 MB Access on demand
519 KB Access on demand
577 KB Access on demand
42 KB Access on demand
189 KB Access on demand
26 KB Access on demand
Top of the page

How to cite 

Boisnoir Aurelie, Bilien Gwenael, Lemée Rodolphe, Chomérat Nicolas (2022). First insights on the diversity of the genus Ostreopsis (Dinophyceae, Gonyaulacales) in Guadeloupe Island, with emphasis on the phylogenetic position of O. heptagona. European Journal Of Protistology, 83, 125875 (21p.). Publisher's official version : , Open Access version :