The Fucoxanthin Chlorophyll a/c-Binding Protein in Tisochrysis lutea: Influence of Nitrogen and Light on Fucoxanthin and Chlorophyll a/c-Binding Protein Gene Expression and Fucoxanthin Synthesis

Type Article
Date 2022-02
Language English
Author(s) Pajot Anne1, Lavaud Johann2, Carrier Gregory1, Garnier Matthieu1, Saint-Jean Bruno1, Rabilloud Noémie1, Baroukh Caroline1, Bérard Jean-Baptiste1, Bernard Olivier3, Marchal Luc4, Nicolau Elodie1
Affiliation(s) 1 : IFREMER, Physiology and Biotechnology of Algae Laboratory, Nantes, France
2 : LEMAR-Laboratoire des Sciences de l’Environnement Marin, UMR 6539, CNRS/Univ Brest/Ifremer/IRD, Institut Universitaire Européen de la Mer, Technopôle Brest-Iroise, Plouzané, France
3 : Université Côte d’Azur, Biocore, INRIA, CNRS, Sorbonne Université (LOV, UMR 7093), Sophia-Antipolis, France
4 : Univ Nantes, GEPEA, Nantes, France
Source Frontiers In Plant Science (1664-462X) (Frontiers Media SA), 2022-02 , Vol. 13 , P. 830069 (17p.)
DOI 10.3389/fpls.2022.830069
WOS© Times Cited 2
Keyword(s) carotenoids, fucoxanthin and chlorophyll a, c-binding proteins, Lhcf, Lhcr, Lhcx, Tisochrysis lutea, turbidostat culture, chemostat culture
Abstract

We observed differences in lhc classification in Chromista. We proposed a classification of the lhcf family with two groups specific to haptophytes, one specific to diatoms, and one specific to seaweeds. Identification and characterization of the Fucoxanthin and Chlorophyll a/c-binding Protein (FCP) of the haptophyte microalgae Tisochrysis lutea were performed by similarity analysis. The FCP family contains 52 lhc genes in T. lutea. FCP pigment binding site candidates were characterized on Lhcf protein monomers of T. lutea, which possesses at least nine chlorophylls and five fucoxanthin molecules, on average, per monomer. The expression of T. lutea lhc genes was assessed during turbidostat and chemostat experiments, one with constant light (CL) and changing nitrogen phases, the second with a 12 h:12 h sinusoidal photoperiod and changing nitrogen phases. RNA-seq analysis revealed a dynamic decrease in the expression of lhc genes with nitrogen depletion. We observed that T. lutea lhcx2 was only expressed at night, suggesting that its role is to protect \cells from return of light after prolonged darkness exposure.

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Pajot Anne, Lavaud Johann, Carrier Gregory, Garnier Matthieu, Saint-Jean Bruno, Rabilloud Noémie, Baroukh Caroline, Bérard Jean-Baptiste, Bernard Olivier, Marchal Luc, Nicolau Elodie (2022). The Fucoxanthin Chlorophyll a/c-Binding Protein in Tisochrysis lutea: Influence of Nitrogen and Light on Fucoxanthin and Chlorophyll a/c-Binding Protein Gene Expression and Fucoxanthin Synthesis. Frontiers In Plant Science, 13, 830069 (17p.). Publisher's official version : https://doi.org/10.3389/fpls.2022.830069 , Open Access version : https://archimer.ifremer.fr/doc/00752/86392/