The Active Microbiota of the Eggs and the Nauplii of the Pacific Blue Shrimp Litopenaeus stylirostris Partially Shaped by a Potential Vertical Transmission

Type Article
Date 2022-05
Language English
Author(s) Giraud Carolane1, 2, Callac Nolwenn1, Boulo Viviane1, Lam Jean-Sebastien1, Pham Dominique1, Selmaoui-Folcher Nazha2, Wabete Nelly1
Affiliation(s) 1 : UMR 9220 ENTROPIE, Ifremer (LEAD-NC), Noumea, New Caledonia
2 : Institut des Sciences Exactes et Appliquées (ISEA), University of New Caledonia, Noumea, New Caledonia
Source Frontiers In Microbiology (1664-302X) (Frontiers Media SA), 2022-05 , Vol. 13 , P. 886752 (13p.)
DOI 10.3389/fmicb.2022.886752
Keyword(s) active microbiota, shrimps, eggs, nauplii, vertical transmission, microbial colonization, core microbiota
Abstract

The many ecological niches present in an organism harbor distinct microorganisms called microbiota. Different factors can influence the establishment of these commensal microbial communities. In a previous article, we have concluded that some bacterial lineages associated with the early larval stages of the Pacific blue shrimp Litopenaeus stylirostris could be acquired from the breeders via a potential vertical transmission. The present study was conducted in order to investigate this hypothesis. Using HiSeq sequencing of the V4 region of 16S rRNA gene, we analyzed the active microbiota associated with the eggs and the nauplii of L. stylirsotris as well as with the reproductive organs of their breeders. Microbial communities associated with the rearing water were also considered to discriminate environmental microbial lineages. Using these analyses, we highlight a set of core bacterial families present in all samples and composed of members of Colwelliaceae, Alteromonadaceae, Pseudoalteromonadaceae, Saccharospirillaceae, Oceanospirillaceae, Vibrionaceae, Burkholderiaceae, Rhodobacteraceae, Flavobacteraceae, and Corynebacteriaceae; showing the importance of the environment in the establishment of the larval microbiota. We also present specific bacteria affiliated to the Arcobacteraceae, Rhodobacteraceae, Comamonadaceae, and Colwelliaceae families, which were only found in the breeders and their offspring strengthening the hypothesis of a potential vertical transmission shaping the active microbiota of the eggs and the nauplii of L. stylirostris.

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Supplementary Figure S1 | Venn diagrams of each sample type. Specific and shared ASVs among all (A) the tank water samples, (B) the female reproductive organ samples, (C) the male reproductive... 171 KB Open access
upplementary Figure S2 | Alpha diversity index of all samples. (A) Chao1 and (B) Simpson index for all the tank waters, the egg, the nauplius (nii), the female and the male samples. Significant ... 163 KB Open access
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How to cite 

Giraud Carolane, Callac Nolwenn, Boulo Viviane, Lam Jean-Sebastien, Pham Dominique, Selmaoui-Folcher Nazha, Wabete Nelly (2022). The Active Microbiota of the Eggs and the Nauplii of the Pacific Blue Shrimp Litopenaeus stylirostris Partially Shaped by a Potential Vertical Transmission. Frontiers In Microbiology, 13, 886752 (13p.). Publisher's official version : https://doi.org/10.3389/fmicb.2022.886752 , Open Access version : https://archimer.ifremer.fr/doc/00770/88198/