Dissemination of Extended-Spectrum-β-Lactamase-Producing Enterobacter cloacae Complex from a Hospital to the Nearby Environment in Guadeloupe (French West Indies): ST114 Lineage Coding for a Successful IncHI2/ST1 Plasmid

Type Article
Date 2021-03
Language English
Author(s) Pot MatthieuORCID1, Guyomard-Rabenirina Stephanie1, Couvin DavidORCID1, Ducat Celia1, Enouf Vincent2, Ferdinand Severine1, Gruel Gaelle1, Malpote Edith3, Talarmin Antoine1, Breurec Sebastien1, 4, 5, Reynaud Yann1
Affiliation(s) 1 : Pasteur Inst Guadeloupe, Transmiss Reservoir & Divers Pathogens Unit, Les Abymes, France.
2 : Pasteur Inst Paris, Plateforme Microbiol Mutualisee P2M, Pasteur Int Bioresources Network PIBnet, Paris, France.
3 : Univ Hosp Guadeloupe, Lab Clin Microbiol, Pointe A Pitre, Guadeloupe, France.
4 : Univ Antilles, Fac Med Hyacinthe Bastaraud, Pointe A Pitre, Guadeloupe, France.
5 : INSERM, Ctr Clin Invest 1424, Pointe A Pitre, Guadeloupe, France.
Source Antimicrobial Agents And Chemotherapy (0066-4804) (Amer Soc Microbiology), 2021-03 , Vol. 65 , N. 3 , P. e02146-20 (12p.)
DOI 10.1128/AAC.02146-20
WOS© Times Cited 6
Keyword(s) Enterobacter cloacae complex, bla(CTX-M-15), IncHI2, one health, ST114, wastewater treatment plant, wildlife
Abstract

Wastewater treatment plants are considered hot spots for antibiotic resistance. Most studies have addressed the impact on the aquatic environment, as water is an important source of anthropogenic pollutants. Few investigations have been conducted on terrestrial animals living near treatment ponds. We isolated extended-spectrum-beta-lactamase Enterobacter cloacae complex-producing strains from 35 clinical isolates, 29 samples of wastewater, 19 wild animals, and 10 domestic animals living in the hospital sewers and at or near a wastewater treatment plant to study the dissemination of clinically relevant resistance through hospital and urban effluents. After comparison of the antibiotic-resistant profiles of E. cloacae complex strains, a more detailed analysis of 41 whole-genome-sequenced strains demonstrated that the most common sequence type, ST114 (n =20), was present in human (n =9) and nonhuman (n = 11) samples, with a close genetic relatedness. Whole-genome sequencing confirmed local circulation of this pathogenic lineage in diverse animal species. In addition, nanopore sequencing and specific synteny of an IncHI2/ST1/bla(CTX-M-15), plasmid recovered on the majority of these ST114 clones (n =18) indicated successful worldwide diffusion of this mobile genetic element.

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Pot Matthieu, Guyomard-Rabenirina Stephanie, Couvin David, Ducat Celia, Enouf Vincent, Ferdinand Severine, Gruel Gaelle, Malpote Edith, Talarmin Antoine, Breurec Sebastien, Reynaud Yann (2021). Dissemination of Extended-Spectrum-β-Lactamase-Producing Enterobacter cloacae Complex from a Hospital to the Nearby Environment in Guadeloupe (French West Indies): ST114 Lineage Coding for a Successful IncHI2/ST1 Plasmid. Antimicrobial Agents And Chemotherapy, 65(3), e02146-20 (12p.). Publisher's official version : https://doi.org/10.1128/AAC.02146-20 , Open Access version : https://archimer.ifremer.fr/doc/00771/88292/