Interactions between infernan and calcium: From the molecular level to the mechanical properties of microgels

Type Article
Date 2022-09
Language English
Author(s) Zykwinska AgataORCID1, Makshakova Olga2, Gélébart Perrine1, Sinquin CorinneORCID1, Stephant Nicolas3, Colliec-Jouault SylviaORCID1, Perez Stella4, Cuenot Stéphane3
Affiliation(s) 1 : Ifremer, MASAE, 44300 Nantes, France
2 : Kazan Institute of Biochemistry and Biophysics, FRC Kazan Scientific Center, RAS, Lobachevsky Str., 2/31, 420111 Kazan, Russian Federation
3 : Nantes Université, CNRS, Institut des Matériaux Jean Rouxel, IMN, Nantes, France
4 : Centre de Recherches sur les Macromolécules Végétales, Université de Grenoble Alpes, Centre National de la Recherche Scientifique, Grenoble, France
Source Carbohydrate Polymers (0144-8617) (Elsevier BV), 2022-09 , Vol. 292 , P. 119629 (11p.)
DOI 10.1016/j.carbpol.2022.119629
WOS© Times Cited 1
Keyword(s) Atomic force microscopy, Molecular dynamics simulations, Persistence length, Viscoelastic properties, Microfluidics

With the increasing need for hydrogels with tunable properties for specific biomedical applications, a complete understanding of the structure-function relationship of polymers used for hydrogel development remains crucial for their optimal use. In the present study, by combining experimental and theoretical approaches, the structure-function relationship of a bacterial exopolysaccharide, infernan, displaying both glycosaminoglycan-mimetic and gelling properties, was investigated at molecular and microscopic levels. Atomic force microscopy (AFM) experiments and molecular dynamics simulations were applied to determine the persistence length of individual infernan chains before studying their association induced by calcium. Infernan-based microgels were then produced using microfluidics and their mechanical properties were characterized by AFM methods. The mechanical properties of EPS/calcium microgels were finely tuned by varying the crosslinking density of their network, either by calcium or EPS concentrations. The obtained set of viscoelastic microgels with different elastic modulus values opens several possibilities for their applications in tissue engineering.

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Zykwinska Agata, Makshakova Olga, Gélébart Perrine, Sinquin Corinne, Stephant Nicolas, Colliec-Jouault Sylvia, Perez Stella, Cuenot Stéphane (2022). Interactions between infernan and calcium: From the molecular level to the mechanical properties of microgels. Carbohydrate Polymers, 292, 119629 (11p.). Publisher's official version : , Open Access version :