Toxicity of palytoxin, purified ovatoxin-a, ovatoxin-d and extracts of Ostreopsis cf. ovata on the Caco-2 intestinal barrier model

Type Article
Date 2022-08
Language English
Author(s) Gemin Marin-Pierre1, Lanceleur Rachelle2, Meslier Lisa2, Herve FabienneORCID1, Reveillon DamienORCID1, Amzil ZouherORCID1, Ternon Eva3, Thomas P.Olivier4, Fessard Valérie2
Affiliation(s) 1 : IFREMER, Phycotoxins Laboratory, F-44311 Nantes, France
2 : ANSES, Fougères Laboratory, Toxicology of Contaminants Unit, French Agency for Food, Environmental and Occupational Health & Safety, Fougères 35306, France
3 : Sorbonne Université, CNRS, Laboratoire d’Océanographie de Villefranche, UMR 709, BP 28, F-06230 Villefranche-sur-Mer, France
4 : Marine Biodiscovery, School of Chemistry and Ryan Institute, National University of Ireland Galway, University Road, H91TK33 Galway, Ireland
Source Environmental Toxicology And Pharmacology (1382-6689) (Elsevier BV), 2022-08 , Vol. 94 , P. 103909 (11p.)
DOI 10.1016/j.etap.2022.103909
WOS© Times Cited 1
Keyword(s) Palytoxin, Ovatoxins, Toxicity, Permeability, Purification, Inflammation

Human intoxications in the Mediterranean Sea have been linked to blooms of the dinoflagellate Ostreopsis cf. ovata, producer of palytoxin (PlTX)-like toxins called ovatoxins (OVTXs). Exposure routes include only inhalation and contact, although PlTX-poisoning by seafood has been described in tropical regions. To address the impact of OVTXs on the intestinal barrier, dinoflagellate extracts, purified OVTX-a and -d and PlTX were tested on differentiated Caco-2 cells. Viability, inflammatory response and barrier integrity were recorded after 24 h treatment. OVTX-a and -d were not cytotoxic up to 20 ng/mL but increased IL-8 release, although to a lesser extent compared to PlTX. While PlTX and OVTX-a (at 0.5 and 5 ng/mL respectively) affected intestinal barrier integrity OVTX-d up to 5 ng/mL did not. Overall, OVTX-d was shown to be less toxic than OVTX-a and PlTX. Therefore, oral exposure to OVTX-a and -d could provoked lower acute toxicity than PlTX.

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Gemin Marin-Pierre, Lanceleur Rachelle, Meslier Lisa, Herve Fabienne, Reveillon Damien, Amzil Zouher, Ternon Eva, Thomas P.Olivier, Fessard Valérie (2022). Toxicity of palytoxin, purified ovatoxin-a, ovatoxin-d and extracts of Ostreopsis cf. ovata on the Caco-2 intestinal barrier model. Environmental Toxicology And Pharmacology, 94, 103909 (11p.). Publisher's official version : , Open Access version :