Natural cortisol production is not linked to the sexual fate of European sea bass
Type | Article | ||||||||||||
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Date | 2022-08 | ||||||||||||
Language | English | ||||||||||||
Author(s) | Goikoetxea Alexander1, Servili Arianna2, Houdelet Camille1, Mouchel Olivier2, Hermet Sophie3, Clota Frederic1, 4, Aerts Johan5, Fernandino Juan Ignacio6, Allal Francois1, Vandeputte Marc1, 4, Blondeau-Bidet Eva3, Geffroy Benjamin1 | ||||||||||||
Affiliation(s) | 1 : MARBEC Univ Montpellier, CNRS, Ifremer, IRD, Palavas-Les-Flots, France 2 : Ifremer, IFREMER, Univ Brest, CNRS, IRD, LEMAR, 29280, Plouzané, France 3 : MARBEC Univ Montpellier, CNRS, Ifremer, IRD, Montpellier, France 4 : Université Paris-Saclay, INRAE, AgroParisTech, GABI, Jouy-en-Josas, France 5 : Stress Physiology Research Group, Faculty of Sciences, Ghent University, Ostend, Belgium 6 : Instituto Tecnológico de Chascomús, INTECH (CONICET-UNSAM), Chascomús, Argentina |
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Source | Fish Physiology And Biochemistry (0920-1742) (Springer Science and Business Media LLC), 2022-08 , Vol. 48 , N. 4 , P. 1117-1135 | ||||||||||||
DOI | 10.1007/s10695-022-01104-1 | ||||||||||||
WOS© Times Cited | 3 | ||||||||||||
Keyword(s) | Sex determination, Sex differentiation, Temperature, Cortisol, European sea bass | ||||||||||||
Abstract | In this study, we aimed to investigate the relationship between cortisol and the determination of sexual fate in the commercially important European sea bass (Dicentrarchus labrax). To test our hypothesis, we designed two temperature-based experiments (19 ℃, 21 ℃ and 23 ℃, experiment 1; 16 ℃ and 21 ℃, experiment 2) to assess the effects of these thermal treatments on European sea bass sex determination and differentiation. In the fish from the first experiment, we evaluated whether blood cortisol levels and expression of stress key regulatory genes were different between differentiating (149 to 183 dph) males and females. In the second experiment, we assessed whether cortisol accumulated in scales over time during the labile period for sex determination as well as the neuroanatomical localisation of brain cells expressing brain aromatase (cyp19a1b) and corticotropin-releasing factor (crf) differed between males and females undergoing molecular sex differentiation (117 to 124 dph). None of the gathered results allowed to detect differences between males and females regarding cortisol production and regulatory mechanisms. Altogether, our data provide strong physiological, molecular and histochemical evidence, indicating that in vivo cortisol regulation has no major effects on the sex of European sea bass. |
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