Common carp (Cyprinus carpio) obtain omega-3 long-chain polyunsaturated fatty acids via dietary supply and endogenous bioconversion in semi-intensive aquaculture ponds

Type Article
Date 2022-12
Language English
Author(s) Pilecky Matthias1, 2, Mathieu-Resuge MargauxORCID1, 3, 4, Závorka Libor1, Fehlinger Lena1, 6, Winter Katharina1, Martin-Creuzburg Dominik5, Kainz Martin J.1, 2
Affiliation(s) 1 : WasserCluster – Biologische Station Lunz, Inter-University Center for Aquatic Ecosystem Research, Dr. Carl-Kupelwieser Promenade 5, 3293 Lunz/See, Austria
2 : Donau-Universität Krems, Department for Biomedical Research, Dr. Karl-Dorrek-Straße 30, 3500 Krems, Austria
3 : University of Brest, CNRS, IRD, Ifremer, LEMAR, F-29280 Plouzane, France
4 : Ifremer, Laboratoire de Biologie Halieutique, ZI Pointe du Diable - CS 10070, 29 280 Plouzané, France
5 : BTU Cottbus-Senftenberg, Department of Aquatic Ecology, Research Station Bad Saarow, Seestraße 45, 15526 Bad Saarow, Germany
6 : University of Vic - Central University of Catalonia, Aquatic Ecology Group, Carrer de la Sagrada Família, 7, 08500 Vic, Barcelona, Spain
Source Aquaculture (0044-8486) (Elsevier BV), 2022-12 , Vol. 561 , P. 738731 (9p.)
DOI 10.1016/j.aquaculture.2022.738731
WOS© Times Cited 5
Keyword(s) Aquaculture, Fatty acid bioconversion, Compound-specific stable isotope analysis, Deuterium, DHA, Fish ecophysiology
Abstract

Omega-3 long-chain polyunsaturated fatty acids (n-3 LC-PUFA) are essential micronutrients for aquatic consumers. Synthesized by aquatic primary producers, n-3 LC-PUFA are transferred across trophic levels and may eventually end up accumulating in fish. However, if short in dietary supply, fish may also biosynthesize n-3 LC-PUFA from dietary precursors (i.e., n-3 C18-PUFA). We applied compound-specific hydrogen stable isotope analysis (CSIA) of fatty acids to investigate sources and metabolic processes of n-3 LC-PUFA, and in particular of docosahexaenoic acid (22:6n-3, DHA), in Common Carp (Cyprinus carpio) raised in semi-intensive aquaculture ponds. Carp were feeding on natural pond zooplankton and benthic macroinvertebrates rich in n-3 LC-PUFA and cereal-based pellet feeds rich in C18-PUFA. Results provide isotopic evidence that carp obtained a significant amount of dietary lipids and nitrogen from added cereal-based feeds, while n-3 LC-PUFA were generally acquired by feeding on benthic macroinvertebrates and zooplankton. However, DHA retained in carp was also generated endogenously via bioconversion from dietary PUFA precursors, such as EPA. DHA was isotopically lighter than EPA and likely not supplied in sufficient quantities to meet the physiological requirements for DHA in carp. Our data show that depending on the natural abundance of dietary DHA in these eutrophic ponds, farmed carp can obtain DHA by two different pathways; i.e., directly via dietary uptake and indirectly via bioconversion. This field study highlights the importance of dietary LC-PUFA supply in eutrophic aquatic ecosystems and the ability of carp to biosynthesize highly valuable LC-PUFA, eventually also benefiting human health.

Full Text
File Pages Size Access
Publisher's official version 27 2 MB Open access
Top of the page

How to cite 

Pilecky Matthias, Mathieu-Resuge Margaux, Závorka Libor, Fehlinger Lena, Winter Katharina, Martin-Creuzburg Dominik, Kainz Martin J. (2022). Common carp (Cyprinus carpio) obtain omega-3 long-chain polyunsaturated fatty acids via dietary supply and endogenous bioconversion in semi-intensive aquaculture ponds. Aquaculture, 561, 738731 (9p.). Publisher's official version : https://doi.org/10.1016/j.aquaculture.2022.738731 , Open Access version : https://archimer.ifremer.fr/doc/00788/90029/