Mitochondrial oxidative phosphorylation response overrides glucocorticoid-induced stress in a reptile

Type Article
Date 2022-11
Language English
Author(s) Voituron YannORCID1, Roussel Damien1, Le Galliard Jean-François2, 3, Dupoué AndreazORCID2, Romestaing Caroline1, Meylan Sandrine2
Affiliation(s) 1 : Laboratoire d’Ecologie des Hydrosystèmes Naturels et Anthropisés (U.M.R. CNRS 5023), Université Claude Bernard Lyon1, Université de Lyon, Bd du 11 novembre 1918, Bât. Darwin C, 69622, Villeurbanne Cedex, France
2 : Sorbonne Université, CNRS, IRD, INRA, Institut d’écologie Et Des Sciences de L’environnement (IEES), Paris, France
3 : Centre de Recherche en Écologie Expérimentale et Prédictive (CEREEP-Ecotron IleDeFrance), Département de biologie, Ecole Normale Supérieure, CNRS, PSL University, 77140, Saint-Pierre-lès-Nemours, France
Source Journal Of Comparative Physiology B-biochemical Systems And Environmental Physiology (0174-1578) (Springer Science and Business Media LLC), 2022-11 , Vol. 192 , N. 6 , P. 765-774
DOI 10.1007/s00360-022-01454-5
WOS© Times Cited 1
Keyword(s) Corticosterone, Lizard, Metabolism, Mitochondrial efficiency, ROS and ATP production, Oxygen consumption

Stress hormones and their impacts on whole organism metabolic rates are usually considered as appropriate proxies for animal energy budget that is the foundation of numerous concepts and models aiming at predicting individual and population responses to environmental stress. However, the dynamics of energy re-allocation under stress make the link between metabolism and corticosterone complex and still unclear. Using ectopic application of corticosterone for 3, 11 and 21 days, we estimated a time effect of stress in a lizard (Zootoca vivipara). We then investigated whole organism metabolism, muscle cellular O2 consumption and liver mitochondrial oxidative phosphorylation processes (O2 consumption and ATP production) and ROS production. The data showed that while skeletal muscle is not impacted, stress regulates the liver mitochondrial functionality in a time-dependent manner with opposing pictures between the different time expositions to corticosterone. While 3 days exposition is characterized by lower ATP synthesis rate and high H2O2 release with no change in the rate of oxygen consumption, the 11 days exposition reduced all three fluxes of about 50%. Oxidative phosphorylation capacities in liver mitochondria of lizard treated with corticosterone for 21 days was similar to the hepatic mitochondrial capacities in lizards that received no corticosterone treatment but with 40% decrease in H2O2 production. This new mitochondrial functioning allows a better capacity to respond to the energetic demands imposed by the environment but do not influence whole organism metabolism. In conclusion, global mitochondrial functioning has to be considered to better understand the proximal causes of the energy budget under stressful periods.

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Voituron Yann, Roussel Damien, Le Galliard Jean-François, Dupoué Andreaz, Romestaing Caroline, Meylan Sandrine (2022). Mitochondrial oxidative phosphorylation response overrides glucocorticoid-induced stress in a reptile. Journal Of Comparative Physiology B-biochemical Systems And Environmental Physiology, 192(6), 765-774. Publisher's official version : , Open Access version :