Sub-micrometric spatial distribution of amorphous and crystalline carbonates in biogenic crystals using coherent Raman microscopy

Type Article
Date 2022-12
Language English
Author(s) Dicko Hamadou1, Grünewald Tilman A.ORCID1, Ferrand PatrickORCID1, Vidal-Dupiol JeremieORCID2, Teaniniuraitemoana Vaihiti3, Sham Koua Manaarii3, Le Moullac GillesORCID3, Le Luyer JeremyORCID3, Saulnier Denis3, Chamard VirginieORCID1, Duboisset Julien1
Affiliation(s) 1 : Aix-Marseille Univ, CNRS, Centrale Marseille, Institut Fresnel, Marseille, France
2 : IHPE, Univ. Montpellier, CNRS, Ifremer, Univ. Perpignan Via Domitia, Montpellier, France
3 : Ifremer, UMR 241 Environnement Insulaire Océanien (EIO), Labex Corail, Centre du Pacifique, BP 49, Vairao 98719, French Polynesia
Source Journal Of Structural Biology (1047-8477) (Elsevier BV), 2022-12 , Vol. 214 , N. 4 , P. 107909 (10p.)
DOI 10.1016/j.jsb.2022.107909
WOS© Times Cited 1
Keyword(s) Coherent Raman, Optical microscopy, Calcium carbonate, Shell oyster, Crystalline transition, Biomineralization
Abstract

In living organisms, calcium carbonate biomineralization combines complex bio-controlled physical and chemical processes to produce crystalline hierarchical hard tissues (usually calcite or aragonite) typically from an amorphous precursor phase. Understanding the nature of the successive transient amorphous phases potentially involved in the amorphous-to-crystalline transition requires characterization tools, which are able to provide a spatial and spectroscopic analysis of the biomineral structure. In this work, we present a highly sensitive coherent Raman microscopy approach, which allows one to image molecular bond concentrations in post mortem shells and living animals, by exploiting the vibrational signature of the different carbonates compounds. To this end, we target the calcium carbonate vibration mode and produce spatially and spectroscopically resolved images of the shell border of a mollusk shell, the Pinctada margaritifera pearl oyster. A novel approach is further presented to efficiently compare the amount of amorphous carbonate with respect to its crystalline counterpart. Finally, the whole microscopy method is used to image in vivo the shell border and demonstrate the feasibility and the reproducibility of the technique. These findings open chemical imaging perspectives for the study of biogenic and bio-inspired crystals.

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Dicko Hamadou, Grünewald Tilman A., Ferrand Patrick, Vidal-Dupiol Jeremie, Teaniniuraitemoana Vaihiti, Sham Koua Manaarii, Le Moullac Gilles, Le Luyer Jeremy, Saulnier Denis, Chamard Virginie, Duboisset Julien (2022). Sub-micrometric spatial distribution of amorphous and crystalline carbonates in biogenic crystals using coherent Raman microscopy. Journal Of Structural Biology, 214(4), 107909 (10p.). Publisher's official version : https://doi.org/10.1016/j.jsb.2022.107909 , Open Access version : https://archimer.ifremer.fr/doc/00800/91212/