Deciphering interactions between the marine dinoflagellate Prorocentrum lima and the fungus Aspergillus pseudoglaucus

Type Article
Date 2023-02
Language English
Author(s) Berry Olivier1, Briand EnoraORCID2, Bagot Alizé1, 2, Chaigné Maud1, 2, Meslet‐cladière Laurence3, Wang Julien1, Grovel Olivier1, Jansen Jeroen J.4, Ruiz Nicolas1, Robiou Du Pont Thibaut1, Pouchus Yves François1, Hess PhilippORCID2, Bertrand Samuel1
Affiliation(s) 1 : Institut des Substances et Organismes de la Mer, ISOMER Nantes Université, UR 2160, Nantes, France
2 : IFREMER, PHYTOX, Nantes, France
3 : Univ Brest, INRAE, Laboratoire Universitaire de Biodiversité et Écologie Microbienne, Plouzané, France
4 : Radboud University, Institute for Molecules and Materials, Heyendaalseweg 135, 6525 AJ Nijmegen, The Netherlands
Source Environmental Microbiology (1462-2912) (Wiley), 2023-02 , Vol. 25 , N. 2 , P. 250-267
DOI 10.1111/1462-2920.16271
WOS© Times Cited 5

The comprehension of microbial interactions is one of the key challenges in marine microbial ecology. This study focused on exploring chemical interactions between the toxic dinoflagellate Prorocentrum lima and a filamentous fungal species, Aspergillus pseudoglaucus, which has been isolated from the microalgal culture. Such interspecies interactions are expected to occur even though they were rarely studied. Here, a co-culture system was designed in a dedicated microscale marine-like condition. This system allowed to explore microalgal-fungal physical and metabolic interactions in presence and absence of the bacterial consortium. Microscopic observation showed an unusual physical contact between the fungal mycelium and dinoflagellate cells. To delineate specialized metabolome alterations during microalgal-fungal co-culture metabolomes were monitored by high-performance liquid chromatography coupled to high-resolution mass spectrometry. In-depth multivariate statistical analysis using dedicated approaches highlighted (1) the metabolic alterations associated with microalgal-fungal co-culture, and (2) the impact of associated bacteria in microalgal metabolome response to fungal interaction. Unfortunately, only a very low number of highlighted features were fully characterised. However, an up-regulation of the dinoflagellate toxins okadaic acid and dinophysistoxin 1 was observed during co-culture in supernatants. Such results highlight the importance to consider microalgal-fungal interactions in the study of parameters regulating toxin production.

Full Text
File Pages Size Access
Publisher's official version 41 6 MB Open access
Appendix S1. Supporting Information 9 947 KB Open access
Top of the page

How to cite 

Berry Olivier, Briand Enora, Bagot Alizé, Chaigné Maud, Meslet‐cladière Laurence, Wang Julien, Grovel Olivier, Jansen Jeroen J., Ruiz Nicolas, Robiou Du Pont Thibaut, Pouchus Yves François, Hess Philipp, Bertrand Samuel (2023). Deciphering interactions between the marine dinoflagellate Prorocentrum lima and the fungus Aspergillus pseudoglaucus. Environmental Microbiology, 25(2), 250-267. Publisher's official version : , Open Access version :