Screening of marine lactic acid bacteria for Vibrio parahaemolyticus inhibition and application to depuration in Pacific oysters (Crassostrea gigas)

Type Article
Date 2023-02
Language English
Author(s) Soree MarionORCID1, Kolypczuk LaetitiaORCID1, Hadjiev Emilie1, Lozach Solen2, Verrez-Bagnis VéroniqueORCID1, Delbarre-Ladrat ChristineORCID1, Hervio Heath DominiqueORCID2, Passerini DelphineORCID1
Affiliation(s) 1 : Ifremer, MASAE Microbiologie Aliment Santé Environnement, F-44000 Nantes, France
2 : Ifremer, Univ Brest, CNRS, IRD, LEMAR, F‐29280 Plouzané, France
Source Journal of Applied Microbiology (1364-5072) (Wiley / Blackwell), 2023-02 , Vol. 134 , N. 2 , P. 1-9
DOI 10.1093/jambio/lxac081
Keyword(s) V. parahaemolyticus, Lactic acid bacteria, flow cytometry, oyster depuration, challenge test, seafood safety
Abstract

Aims

This study aims to assess the use of marine lactic acid bacteria (LAB) to reduce V. parahaemolyticus levels during oyster depuration process.

Methods and Results

The inhibitory effect of thirty marine LAB strains against V. parahaemolyticus strains was evaluated by in vitro assays. Three positive strains (Latilactobacillus sakei SF1583, Lactococcus lactis SF1945 and Vagococcus fluvialis CD264) were selected for V. parahaemolyticus levels reduction during oyster depuration. Pacific oysters Crassostrea gigas were artificially and independently contaminated by four GFP-labelled V. parahaemolyticus strains (IFVp201, IFVp69, IFVp195 and LMG2850T) at 105 CFU.mL−1 and then exposed by balneation to 106 CFU.mL−1 of each LAB strains during 24 h, at 19°C. Quantification of V. parahaemolyticus in haemolymph by flow cytometry revealed variations in natural depuration of the different V. parahaemolyticus strains alone. Furthermore, the addition of LABs improved up to 1-log bacteria.mL−1 the reduction of IFVp201 concentration in comparison to the control condition.

Conclusions

Although further optimizations of procedure are needed, addition of marine LABs during oyster depuration may be an interesting strategy to reduce V. parahaemolyticus levels in C. gigas.

Significance and impact of the study

Our study provides promising ways to develop a depuration process which could potentially be implemented in oyster farms.

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How to cite 

Soree Marion, Kolypczuk Laetitia, Hadjiev Emilie, Lozach Solen, Verrez-Bagnis Véronique, Delbarre-Ladrat Christine, Hervio Heath Dominique, Passerini Delphine (2023). Screening of marine lactic acid bacteria for Vibrio parahaemolyticus inhibition and application to depuration in Pacific oysters (Crassostrea gigas). Journal of Applied Microbiology, 134(2), 1-9. Publisher's official version : https://doi.org/10.1093/jambio/lxac081 , Open Access version : https://archimer.ifremer.fr/doc/00813/92449/