Molecular and phenotypic effects of early exposure to an environmentally relevant pesticide mixture in the Pacific oyster, Crassostrea gigas.
Type | Article | ||||||||||||||||||||
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Date | 2023-06 | ||||||||||||||||||||
Language | English | ||||||||||||||||||||
Author(s) | Sol Dourdin Thomas1, Rivière Guillaume2, Cormier Alexandre3, Di Poi Broussard Carole4, Guyomard Killian5, Rabiller Manuella4, Akcha Farida1, Bah Thierno Sadialiou1, Le Monier Pauline1, Sussarellu Rossana6 | ||||||||||||||||||||
Affiliation(s) | 1 : Ifremer, Unité Contamination Chimique des Ecosystèmes Marins, Nantes, France 2 : Biologie des Organismes et Ecosystèmes Aquatiques (BOREA), UMR8067, Muséum National D’Histoire Naturelle (MNHN), Centre National de La Recherche Scientifique (CNRS), Institut de Recherche et Développement (IRD), Sorbonne Université (SU), Université de Caen Normandie (UCN), Université des Antilles (UA), 75231, Paris, CEDEX, France 3 : Ifremer, Service de Bioinformatique de L’Ifremer, Brest, France 4 : Laboratoire des Sciences de L'environnement Marin (LEMAR), France 5 : Ifremer, Plateforme Mollusques Marins Bouin, Bouin, France 6 : Ifremer, Physiologie et Toxines des Microalgues Toxiques, Nantes, France |
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Source | Environmental Pollution (0269-7491) (Elsevier BV), 2023-06 , Vol. 326 , P. 121472 (11p.) | ||||||||||||||||||||
DOI | 10.1016/j.envpol.2023.121472 | ||||||||||||||||||||
WOS© Times Cited | 4 | ||||||||||||||||||||
Keyword(s) | Mixture, Epigenetics, Gene expression, Molluscs, Pesticides, DNA methylation | ||||||||||||||||||||
Abstract | Early life stages are crucial for organism development, especially for those displaying external fertilization, whose gametes and early stages face environmental stressors such as xenobiotics. The pacific oyster, Crassostrea gigas, is considered a model species in ecotoxicology because of its ecological characteristics (benthic, sessile, filter feeding). So far studies have investigated the impact of xenobiotics at embryotoxic, genotoxic and physiological endpoints, sometimes at the multigeneration scale, highlighting the role of epigenetic mechanisms in transmitting alterations induced by exposure to single xenobiotics. However, to date, little is known about the impact of environmentally-mimicking contaminants cocktails. Thus, we examined the impact of an early exposure to environmentally relevant mixture on the Pacific oyster life history. We studied transcriptomic, epigenetic and physiological alterations induced in oysters exposed to 18 pesticides and metals at environmental concentration (nominal sum concentration: 2.85 μg.L−1, measured sum concentration: 3.74 ± 0.013 μg.L−1) during embryo-larval stage (0–48 h post fertilization, hpf). No significant differences in embryo-larval abnormalities at 24 hpf were observed during larval and spat rearing; the swimming behaviour of exposed individuals was disturbed, while they were longer and heavier at specific time points, and exhibited a lower epinephrine-induced metamorphosis rate as well as a higher survival rate in the field. In addition, RNA-seq analyses of gastrula embryos revealed the differential expression of development-related genes (e.g. Hox orthologues and cell cycle regulators) between control and exposed oysters. Whole-genome DNA methylation analyses demonstrated a significant modification of DNA methylation in exposed larvae marked by a demethylation trend. Those findings suggest that early exposure to an environmentally relevant pesticide mixture induces multi-scale latent effects possibly affecting life history traits in the Pacific oyster. |
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