Effect of Early Peptide Diets on Zebrafish Skeletal Development

Type Article
Date 2023-04
Language English
Author(s) Printzi Aliki1, 2, Koumoundouros GeorgeORCID1, Fournier Vincent3, Madec Lauriane2, Zambonino-Infante Jose-LuisORCID2, Mazurais DavidORCID2
Affiliation(s) 1 : Biology Department, University of Crete, 70013 Crete, Greece
2 : IFREMER, University of Brest, CNRS, IRD, LEMAR, F-29280 Plouzané, France
3 : DianaAqua, Symrise Group, 56250 Elven, France
Source Biomolecules (2218-273X) (MDPI AG), 2023-04 , Vol. 13 , N. 4 , P. 659 (15p.)
DOI 10.3390/biom13040659
WOS© Times Cited 3
Note This article belongs to the Special Issue Fish Model: Molecular and Cellular Basis of Bone Development and Homeostasis
Keyword(s) nutrition, peptides, skeletal development, haemal lordosis, zebrafish, gene markers

Incorporation of dietary peptides has been correlated with decreased presence of skeletal abnormalities in marine larvae. In an attempt to clarify the effect of smaller protein fractions on fish larval and post-larval skeleton, we designed three isoenergetic diets with partial substitution of their protein content with 0% (C), 6% (P6) and 12% (P12) shrimp di- and tripeptides. Experimental diets were tested in zebrafish under two regimes, with inclusion (ADF-Artemia and dry feed) or lack (DF-dry feed only) of live food. Results at the end of metamorphosis highlight the beneficial effect of P12 on growth, survival and early skeletal quality when dry diets are provided from first feeding (DF). Exclusive feeding with P12 also increased the musculoskeletal resistance of the post-larval skeleton against the swimming challenge test (SCT). On the contrary, Artemia inclusion (ADF) overruled any peptide effect in total fish performance. Given the unknown species’ larval nutrient requirements, a 12% dietary peptide incorporation is proposed for successful rearing without live food. A potential nutritional control of the larval and post-larval skeletal development even in aquaculture species is suggested. Limitations of the current molecular analysis are discussed to enable the future identification of the peptide-driven regulatory pathways.

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