Comparative Analysis of Campylobacter jejuni and C. coli Isolated from Livestock Animals to C. jejuni and C. coli Isolated from Surface Water Using DNA Sequencing and MALDI-TOF

Type Article
Date 2023-09
Language English
Author(s) Denis Martine1, Rose Valérie1, Nagard Bérengère1, Thépault Amandine1, Lucas Pierrick2, Meunier Meagan3, Benoit Fabienne3, Wilhem Amandine4, Gassilloud Benoit4, Cauvin Elodie3, Rincé AlainORCID5, Gourmelon MicheleORCID6
Affiliation(s) 1 : Ploufragan-Plouzané-Niort Laboratory, Hygiene and Quality of Poultry and Pork Products Unit, ANSES (French Agency For Food, Environmental and Occupational Health and Safety), 22440 Ploufragan, France
2 : Ploufragan-Plouzané-Niort Laboratory, Viral Genetics and Biosafety Unit, ANSES, 22440 Ploufragan, France
3 : Caen-Saint-Lô Laboratory, Research Department, LABEO, 50000 Saint-Lô, France
4 : Nancy Laboratory, ANSES, PTF Maldi, 54000 Nancy, France
5 : Bacterial Communication and Anti-Infectious Strategies Reseach Unit, UNICAEN (Caen Normandie University), UR4312 CBSA, 14000 Caen, France
6 : ODE-DYNECO-PELAGOS (Department of Oceanography and Ecosystem Dynamics, Coastal Environment Dynamics and Pelagic Ecology Research Unit), IFREMER (French Research Institute for Exploitation of the Sea), 29280 Plouzané, France
Source Pathogens (2076-0817) (MDPI AG), 2023-09 , Vol. 12 , N. 9 , P. 1069 (16p.)
DOI 10.3390/pathogens12091069
Note This article belongs to the Special Issue Pathogens in 2023
Keyword(s) Campylobacter, livestock animals, surface water, MLST, cgMLST, structure, MALDI-TOF
Abstract

This study evaluated the contribution of cattle, sheep, poultry and pigs to the contamination of surface water from rivers by Campylobacter jejuni and C. coli using MLST, cgMLST and considered MALDI-TOF MS as an alternative technique. The 263 strains isolated from cattle (n = 61), sheep (n = 42), poultry (n = 65), pigs (n = 60) and surface water (n = 35) were distributed across 115 sequence types (STs), 49 for C. jejuni and 66 for C. coli. Considering MLST data, 14.2%, 11.4% and 2.8% of the surface water strains could be attributed to cattle, poultry and sheep, respectively, none to pigs, and 85.7% were non-attributed. Analysis of cg-MLST data with STRUCTURE indicated that C. jejuni strains from water were predominantly attributed to poultry (93.5%), weakly to sheep (<1%) and 6.3% non-attributed, and that conversely, C. coli strains from water were predominantly non-attributed (94.3%) and 5.7% attributed to poultry. Considering the protein profiles with a threshold of 94% and 97% of similarity, respectively, strains from surface water could be attributed to poultry (31.4% and 17.1%), and to cattle (17.1% and 5.7%); 54.1% and 77.1% were non-attributed. This study confirmed these livestock animals might contribute to the contamination of surface water, with a level of contribution depending on the typing technique and the method of analysis. MALDI-TOF could potentially be an alternative approach for source attribution.

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Denis Martine, Rose Valérie, Nagard Bérengère, Thépault Amandine, Lucas Pierrick, Meunier Meagan, Benoit Fabienne, Wilhem Amandine, Gassilloud Benoit, Cauvin Elodie, Rincé Alain, Gourmelon Michele (2023). Comparative Analysis of Campylobacter jejuni and C. coli Isolated from Livestock Animals to C. jejuni and C. coli Isolated from Surface Water Using DNA Sequencing and MALDI-TOF. Pathogens, 12(9), 1069 (16p.). Publisher's official version : https://doi.org/10.3390/pathogens12091069 , Open Access version : https://archimer.ifremer.fr/doc/00849/96141/