Processing of matched and mismatched rNMPs in DNA by archaeal ribonucleotide excision repair
Type | Article | ||||||||||||
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Date | 2023-12 | ||||||||||||
Language | English | ||||||||||||
Author(s) | Reveil Maurane1, Chapel Lucie1, Vourc'h Blandine1, Bossé Audrey1, Vialle Lea1, Brizard Raphael1, Moalic Yann2, Jebbar Mohamed2, Henneke Ghislaine1 | ||||||||||||
Affiliation(s) | 1 : Univ Brest, CNRS, Ifremer, UMR6197 Biologie et Ecologie des Ecosystèmes marins Profonds, 1625 Rte de Sainte-Anne 29280 Plouzané, France 2 : Univ Brest, CNRS, Ifremer, UMR6197 Biologie et Ecologie des Ecosystèmes marins Profonds, 1625 Rte de Sainte-Anne 29280 Plouzané, France |
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Source | Iscience (2589-0042) (Elsevier BV), 2023-12 , Vol. 26 , N. 12 , P. 108479 (19p.) | ||||||||||||
DOI | 10.1016/j.isci.2023.108479 | ||||||||||||
Abstract | Ribonucleoside monophosphates (rNMPs) are the main non-canonical nucleotides in genomic DNA, and their incorporation can occur as mismatches or matches in vivo. To counteract the mutagenic potential of rNMPs in DNA, all organisms evolved ribonucleotide excision repair (RER), a mechanism initiated by type 2 RNase H. Here, we describe the in vitro reconstitution of matched and mismatched rNMP repair using archaeal RER enzymes. Our data suggest two types of RER pathways, including the classical flap RER and a back-up RER with the order of reactions changed for Fen1 and Pols. The genomic rNMP level in RER-deficient or PolB-deficient archaeal cells along with in vitro reconstitution of RER suggest an in vivo role of PolD in RER. Our results provide insights into how matched and mismatched rNMPs may be processed by RER. |
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