Phage-inducible chromosomal minimalist islands (PICMIs), a novel family of small marine satellites of virulent phages

Type Article
Date 2024-01
Language English
Author(s) Barcia-Cruz Rubén1, 2, Goudenège David1, 3, Moura De Sousa Jorge A.4, Piel Damien1, 3, Marbouty MartialORCID5, Rocha Eduardo P. C.4, Le Roux FrederiqueORCID1, 3, 6
Affiliation(s) 1 : Sorbonne Université, CNRS, UMR 8227, Integrative Biology of Marine Models, Station Biologique de Roscoff, CS 90074, F-29688, Roscoff cedex, France
2 : Department of Microbiology and Parasitology, CIBUS-Faculty of Biology, Universidade de Santiago de Compostela, Santiago de Compostela, Spain
3 : Ifremer, Unité Physiologie Fonctionnelle des Organismes Marins, ZI de la Pointe du Diable, CS 10070, F-29280, Plouzané, France
4 : Institut Pasteur, Université Paris Cité, CNRS UMR3525, Microbial Evolutionary Genomics, Paris, France
5 : Institut Pasteur, Université Paris Cité, Organization and Dynamics of Viral Genomes Group, CNRS UMR 3525, Paris, F-75015, France
6 : Département de microbiologie, infectiologie et immunologie, Université de Montréal, Montréal, Canada
Source Nature Communications (2041-1723) (Springer Science and Business Media LLC), 2024-01 , Vol. 15 , N. 1 , P. 664 (13p.)
DOI 10.1038/s41467-024-44965-1
WOS© Times Cited 1

Phage satellites are bacterial genetic elements that co-opt phage machinery for their own dissemination. Here we identify a family of satellites, named Phage-Inducible Chromosomal Minimalist Islands (PICMIs), that are broadly distributed in marine bacteria of the family Vibrionaceae. A typical PICMI is characterized by reduced gene content, does not encode genes for capsid remodelling, and packages its DNA as a concatemer. PICMIs integrate in the bacterial host genome next to the fis regulator, and encode three core proteins necessary for excision and replication. PICMIs are dependent on virulent phage particles to spread to other bacteria, and protect their hosts from other competitive phages without interfering with their helper phage. Thus, our work broadens our understanding of phage satellites and narrows down the minimal number of functions necessary to hijack a tailed phage.

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Barcia-Cruz Rubén, Goudenège David, Moura De Sousa Jorge A., Piel Damien, Marbouty Martial, Rocha Eduardo P. C., Le Roux Frederique (2024). Phage-inducible chromosomal minimalist islands (PICMIs), a novel family of small marine satellites of virulent phages. Nature Communications, 15(1), 664 (13p.). Publisher's official version : , Open Access version :