Structuring effects of archaeal replication origins
Type | Article | ||||||||
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Acceptance Date | 2023-11-15 IN PRESS | ||||||||
Language | English | ||||||||
Author(s) | Mottez Clémence1, Puech Romain1, Flament Didier2, Myllykallio Hannu1 | ||||||||
Affiliation(s) | 1 : Laboratoire d’Optique et Biosciences (CNRS UMR7645, INSERM U1182), Ecole Polytechnique, Institut polytechnique de Paris, F-91128, Palaiseau, France 2 : Laboratoire de Microbiologie des Environnements Extrêmes (PDG-REM-BEEP-LMEE), IFREMER, Centre Bretagne, F-29280, Plouzané, France |
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Source | bioRxiv (Cold Spring Harbor Laboratory) In Press | ||||||||
DOI | 10.1101/2023.11.15.567178 | ||||||||
Note | This article is a preprint and has not been certified by peer review | ||||||||
Abstract | Archaea use eukaryotic-like DNA replication proteins to duplicate circular chromosomes similar to those of bacteria. Although archaeal replication origins have been maintained during the evolution, they are non-essential under laboratory conditions. Here we propose the local deviations from Chargaff’s second parity rule of archaeal chromosomes result from the biased gene orientation and not from mutational biases. Our computational and experimental analyses indicate that the archaeal replication origins prevent head-to-head collisions of replication and transcription complexes as well as participate in coordination of the transfer of genetic information. Our results therefore suggest that the archaeal replication origins have alternative functions not related to their role in initiation of DNA replication. |
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