Recent estimates of the global inventory of dissolved oxygen (DO) have suggested a decrease of 2 % since the 1960s. However, due to the sparse historical oxygen data coverage, the DO inventory exhibits large regional uncertainties at interannual timescale. Using ISASO2, a new DO Argo-based optimally interpolated climatology https://doi.org/10.17882/52367 (Kolodziejczyk et al.,2021), we have estimated an updated regional oxygen inventory. Over the long term (~1980–2013), comparing the ISASO2 Argo fields with the first guess WOA18 built from the DO bottle samples fields extracted from WOD18, the broad tendency to global ocean deoxygenation remains robust in the upper 2000 m with -451±243 Tmol per decade. The oxygen decline is more pronounced in the key ventilation areas of the Southern Ocean and North Atlantic, except in the Nordic Seas, where oxygen has increased. Over the shorter timescale of the Argo period (2005–2019), the deoxygenation tendency seems globally amplified (-1211±218 Tmol per decade). However, DO changes exhibit stronger amplitude and contrasted regional patterns, likely driven by interannual modes of variability in regions as, for instance, the North Atlantic Subpolar-gyre. The recent changes in Apparent Oxygen Utilization mainly explain the interannual variability in the ventilation regions. However, Argo DO coverage is still incomplete at the global and calibration method development are still in progress. Continuing the monitoring of the seasonal to interannual and regional to global DO variability from ISASO2 will improve our ability to reduce uncertainties on global and regional DO inventory.