MiRNAs are single-stranded short noncoding sequences which display crucial roles on gene transcription regulation in many biological processes especially such as embryonic development, cell proliferation or apoptosis. Also, they are recognized for triggering the host’s internal defence mechanisms and immune cell responses thereby playing crucial role in host-parasite interactions. In the present study, the entire and constitutive set of miRNAs, referred to as the miRNome, has been described from the hemolymph, the main immune-related compartment of Biomphalaria glabrata snails, one of the intermediate host of the trematode parasite Schistosoma mansoni, the causative agent of schistosomiasis. A high throughput sequencing approach of small RNAs has revealed the presence of 63 miRNAs in the hemolymphatic compartment. Mollusc-specific miRNAs including bgl-miR-1985-5p and bgl-miR-1984-5p were identified, along with 25 novel miRNAs. Bioinformatic predictions thanks to multiple software tools, help us to identify more than 6000 potential miRNA target gene candidates. Among them was BgTEP1, a complement-like factor involved in parasite clearance. Interestingly, this factor appeared to be targeted by a newly identified miRNA, named bgl-miR-22707-5p. Our study underscores the inherent diversity of miRNAs in the hemolymph of B. glabrata, and discusses their potential role in the regulation of the snail’s innate immune response.