One Health is a term increasingly in focus on a global scale. The One Health concept grew from a centuries-old understanding of the connectedness of human and animal health and the need to consider them together. This is not only the case in the interaction between humans and warm-blooded animals but also related to contact with cold-blooded animals like fish and shellfish, produced for food, or as pet or laboratory animals. During this three hour virtual workshop held during the 21st EAFP Conference, at Aberdeen, Scotland, we aimed at highlighting a One Health approach regarding the interactions of cultured and ornamental fish, shellfish, their environment, pathogens, human health, and human sociological and economic activity, contact- and food-zoonoses by bacteria and parasites, and AMR issues. In total, more than 65 participants from various countries from all over the world participated in this workshop. This paper provides a summary of each presentation and some of the discussions arising from them, giving a snapshot of different One Health perspectives being pursued by the participants.