||Labreuche Yannick2, Soudant Philippe1, Goncalves Madeleine1, Lambert Christophe1, Nicolas Jean-Louis2
||1 : Univ Bretagne Occidentale, Inst Univ Europeen de la Mer, Lab Sci Environm Marin, F-29280 Plouzane, France.
2 : IFREMER, Ctr Brest, Unite Mixte Rech Physiol & Ecophysiol Mollusques, F-29280 Plouzane, France.
||Developmental & Comparative Immunology (0145-305X) (Elsevier), 2006 , Vol. 30 , N. 4 , P. 367-379
|WOS© Times Cited
||Vibrio aestuarianus, ECPs, Crassostrea gigas, ROS production, Adhesion, Phagocytosis, Flow cytometry, Bivalve immunity
||Vibrio aestuarianus strain 01/32 was previously shown to be pathogenic to Crassostrea gigas juveniles. To investigate virulence mechanisms of this pathogen, we studied the toxicity to oysters of its extracellular products (ECPs). ECPs displayed lethality to animals, with a LD50 value of 3.3 mu g/g body weight. To determine the oyster cellular immune responses induced by these ECPs, we further examined in vitro their effects on C. gigas hemocytes, using flow cytometric-based hemocyte assays. Treatment of hemolymph with ECPs caused a significant inhibition of hemocyte phagocytosis and adhesive capabilities. In contrast, the pathway of reactive oxygen species production was enhanced by higher ECP concentrations. Exposure of hemocytes to live bacteria induced no changes in hemocyte parameters. Together, these results suggest that V. aestuarianus strain 01/32 secretes one or more factors which may play an important role in the pathogenicity of this microorganism, and which display immunosuppressant activities on hemocyte functions. (c) 2005 Elsevier Ltd. All fights reserved.