Toxic factors of Vibrio strains pathogenic to shrimp

Type Article
Date 2000-03
Language English
Author(s) Goarant Cyrille1, Herlin Jose1, Brizard Raphael1, Marteau Anne-Laure1, Martin C2, Martin B2
Affiliation(s) 1 : IFREMER, Lab Rech Aquacole, Stn Aquaculture St Vincent, Noumea 98845, New Caledonia.
2 : Univ Francaise Pacifique, Ctr Univ Polynesie Francaise, Biol Cellulaire Lab, Tahiti, Fr Polynesia.
Source Diseases Of Aquatic Organisms (0177-5103) (Inter-research), 2000-03 , Vol. 40 , N. 2 , P. 101-107
DOI 10.3354/dao040101
WOS© Times Cited 34
Keyword(s) vibrio, Vibrio penaeicida, shrimp, extracellular products, temperature
Abstract Vibriosis is a major disease problem in shrimp aquaculture. 'Syndrome 93' is a seasonal juvenile vibriosis caused by Vibrio penaeicida which affects Litopenaeus stylirostris in grow-out ponds in New Caledonia. This study assessed the toxic activities of extracellular products (ECPs) from V: penaeicida, V. alginolyticus and V, nigripulchritudo using in vivo injections in healthy juvenile L. stylirostris (= Penaeus stylirostris) and in vitro assays on shrimp primary cell cultures and the fish cell line epithelioma papulosum cyprini (EPC). Toxic effects of ECPs were demonstrated for all pathogenic Vibrio strains tested both in vivo and in vitro, but for shrimp only; no effect was observed on the fish cell line. ECP toxicity for New Caledonian V. penaeicida was found only after cultivation at low temperature (20 degrees C) and not at higher temperature (30 degrees C). This points to the fact that 'Syndrome 93' episodes are triggered by temperature drops. The assays used here demonstrate the usefulness of primary shrimp cell cultures to study virulence mechanisms of shrimp pathogenic bacteria.
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